| Project/Area Number |
07670947
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
YONEDA Kozo Department of Dermatology, KYOTO UNIVERSITY,assistant, 医学研究科, 助手 (60260626)
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| Co-Investigator(Kenkyū-buntansha) |
太田 敬治 京都大学, 大学院・医学研究科, 助手 (40252431)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Loricrin / Keratinizing disorder / Transglutaminase 1 |
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| Research Abstract |
Loricrin is a major constituent of cornified cell envelope (CE). Loricrin appears at keratohyalin granules at first and is subsequently incorporated into CE.By the way, amongst various hereditary keratinizing disorders, the point mutations of responsible genes were identified only in bullous congenital ichthyosiform erythroderma, some types of lamellar ichthyosis, some types of palmoplantar keratoderma, Sjogren-Larsson syndrome and Vohwinkel syndrome. Vohwinkel syndrome is now thought to be due to the point mutation of loricrin gene. Because loricrin is the major constituent of CE,to understand the expression of loricrin gene in normal and abnormal keratinizing conditions not only facilitates the diagnosis and therapy of Vohwinkel syndrome but also encourages the deep understanding of wide variety of keratinizing disorders. We cultured FRSK cells, normal human epidermal kerationcytes (NHEK), Pam 212 and HaCaT cells in high calcium medium and checked whether CE was formed or not. We found HaCaT cells cultured in high calcium medium could form CE.We also checked the expression of loricrin and transglutaminase 1. It is well known that loricrin is hardly detectable in NHEK cells. In contrast loricrin is abundantly expressed in HaCaT cells cultured in high calcium medium. Thus HaCaT cells cultured in high calcium medium resemble in vivo epidermis. Thus HaCaT cells are very suitable for the research in the expression of loricrin and epidermal keratinization.
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