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The expression of human loricrin gene

Research Project

Project/Area Number 07670947
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

YONEDA Kozo  Department of Dermatology, KYOTO UNIVERSITY,assistant, 医学研究科, 助手 (60260626)

Co-Investigator(Kenkyū-buntansha) 太田 敬治  京都大学, 大学院・医学研究科, 助手 (40252431)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsLoricrin / Keratinizing disorder / Transglutaminase 1
Research Abstract

Loricrin is a major constituent of cornified cell envelope (CE). Loricrin appears at keratohyalin granules at first and is subsequently incorporated into CE.By the way, amongst various hereditary keratinizing disorders, the point mutations of responsible genes were identified only in bullous congenital ichthyosiform erythroderma, some types of lamellar ichthyosis, some types of palmoplantar keratoderma, Sjogren-Larsson syndrome and Vohwinkel syndrome. Vohwinkel syndrome is now thought to be due to the point mutation of loricrin gene. Because loricrin is the major constituent of CE,to understand the expression of loricrin gene in normal and abnormal keratinizing conditions not only facilitates the diagnosis and therapy of Vohwinkel syndrome but also encourages the deep understanding of wide variety of keratinizing disorders. We cultured FRSK cells, normal human epidermal kerationcytes (NHEK), Pam 212 and HaCaT cells in high calcium medium and checked whether CE was formed or not. We found HaCaT cells cultured in high calcium medium could form CE.We also checked the expression of loricrin and transglutaminase 1. It is well known that loricrin is hardly detectable in NHEK cells. In contrast loricrin is abundantly expressed in HaCaT cells cultured in high calcium medium. Thus HaCaT cells cultured in high calcium medium resemble in vivo epidermis. Thus HaCaT cells are very suitable for the research in the expression of loricrin and epidermal keratinization.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Steinert,P.M.: "Expression of transglutaminase l in keratinocytes" J Invest Dermatol. 106. 802-803 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiyama,M.: "Comified cell envelope proteins and keratins are normally distrbuted in harlequin ichthyosis" J Cutan Pathol. 23. 571-575 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiyama,M.: "Expression of transglutaminase l (transglutaminase K) in harlequin idthyosis" Arch Dermatol Res. 289. 116-119 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 米田耕造: "HaCaT細胞cornified cell envelope (CE) 形成過程におけるトランスグルタミナーゼ1の関与" 第11回角化症研究会記録集. PP.44-46 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 秋山真志: "水疱型先天性魚鱗癬様紅皮症患者皮膚の超微形態学的、免疫組織化学的検討" 第11回角化症研究会記録集. PP.10-12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoneda,K.: "Small proline-rich proleins in hair follicles" Acta Derm Venereol (Stockh). 77. 76 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Steinert, P.M.et al: "Expression of transglutaminase 1 in keratinocyies" J Invest Dermatol. 106. 802-803 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiyama, M.et al: "Cornified cell envelope proteins and keratins are normally distributed in harlequin ichthyosis" J Cutan Pathol. 23. 571-575 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiyama, M.et al: "Expression of transglutaminase I (transglutaminasek) in harlequin ichthyosis" Arch Dermatol Res. 289. 116-119 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoneda, K.et al: "Small proline-rich proteins in hair follicles" Acta Derm Venereol. 77. 76 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Steineit,P.M.: "Expression of transglutaminase 1 in keratinocytes." J.Invest.Dermatol.106. 802-803 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akiyama,M.: "Cornified cell envelope proteins and keratins are normally distributed in harlequin ichthyosis." J.Cutan.Pathol.23. 571-575 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akiyama,M.: "Expression of transglutaminase 1 (transglutaminase K) in harlequin ichthyosis." Arch.Dermatol.Res.289. 116-119 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 米田構造: "トランスグルタミナーゼ1のCell Envelope形成における役割" 第11回角化症研究会記録集(スタンダード・マッキンタイヤ), 44-46 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 秋山真志: "水疱型先天性魚鱗癬様紅皮症患者皮膚の超微形態学的、免疫組織化学的検討" 第11回角化症研究会記録集(スタンダード・マッキンタイヤ), 10-12 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoneda, K.: "The human loricrin gene" J. Biol. Chem.267. 18060-18 066 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoneda, K.: "The cornified cell envelope: loricrin and transglutaminases" J. Dermatol.19. 761-764 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoneda, K.: "The over-expression of human loricrin in transgenic mice produces a normal phenotype" Proc. Natl. Acad. Sci. USA. 90. 10754-10758 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoneda, K.: "Atopic retinal detachment" Br. J. Dermatol.133. 586-591 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kim, S. -Y.: "Expression of transglutaminase1 in human epidermis" J. Invest. Dermatol.104. 211-217 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Steinert, P. M.: "Expression of transglutaminase1 in keratinocyte" J. Invest. Dermatol.(in press).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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