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Neuropeptides and phorbolester-induced skin tumor promotion

Research Project

Project/Area Number 07670960
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionOSAKA CITY UNIVERSITY

Principal Investigator

KONO Takeshi  Osaka City University, Medical school, Lecturer, 医学部, 講師 (50170197)

Co-Investigator(Kenkyū-buntansha) MIZUNO Nobuyuki  Osaka City University, Medical school, Lecturer, 医学部, 講師 (80271188)
谷口 彰治  大阪市立大学, 医学部, 講師 (70271187)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
KeywordsTumor promotion / Neuropeptides / carcinogenesis / 実験的皮膚発癌
Research Abstract

We investigated relations of some neuropeptides (NP) and tumor promotion process in the skin. NPs used were substance P (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal peptide (VIP), nerve growth factor (NGF), beta-endorphine (beta-EP) and somatostatin (SOM). As a tumor promotional stimulation. 12-o-tetradecanoypholbol-13-acetate (TPA) was applied to the guinea pig skin.
TPA treatment had no effect on tissue or blood concentration of CGRP,VIP,NGF,SOM.SP concentation increased in the tissue in 6 hr after TPA treatment, and in the blood within 12 hr. beta-EP concentration increased only in the blood in 6 hr.
As a next step, interrelations between NPs and ornithine decarboxylase (ODC), a marker enzyme for tumor promotion process, were investigated. NGF or beta-EP had no effect on TPA-induced ODC activity. On the other hand, SP,CGRP and SOM suppressed TPA-induced ODC activity by about 60% at 3,6 and 9 hrs after TPA treatment. VIP showed biphasic action. VIP pre-treatment stimulated ODC activity, but VIP post-treatment suppressed ODC activity. Any NP had no effect on ODC mRNA level and TPA-stimulated keratinocyte proliferation in tissue section.
These results suggest interactions between TPA-induced tumor promotional process in the skin and NPs.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 幸野 健、水野 信之: "各種神経ペプチドと腫瘍プロモーション" 皮膚(Skin Research). 40. 未定 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kono Takeshi, Mizuno Nobuyuki: Neuropeptides and phorbolester-induced skin tumor promotion. 40(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 幸野 健,水野信之: "各種神経ペプチドと腫瘍プロモーション" 皮膚(Skin Research). 40(未定). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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