Effects of Mutant Keratin Genes in Transfected Epidermal Cells : Establishment of Cell Models for Genodermatoses
| Project/Area Number |
07670966
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | JUNTENDO UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
MANABE Motomu Department of Dermatology, Juntendo University, School of Medicine, Associate Professor, 医学部, 助教授 (30138309)
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| Co-Investigator(Kenkyū-buntansha) |
YAGUCH Hitoshi Department of Dermatology, Juntendo University, School of Medicine, Assistant Pr, 医学部, 講師 (60191095)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | keratins / keratin diseases / transfection |
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| Research Abstract |
To define the interactions required for the filament assembly of differentiation-specific keratins, active copies of mouse hair keratin mHa1 and mHb4 genes were introduced into a rat kangaroo kidney epithelial cell line (PtK2) and a rat stratified squamous epithelial cell line (rat epidermal keratinocyte). In PtK2 transient transfectants when introduced individually or in combination, mHa1 and mHb4 formed aggregates of ring-like structures of various sizes at the perinuclear region with no evidence of organization into a keratin network. These aggregates altered the distribution of the endogenous keratins and vimentin. In most of the cells carrying the ring-like structures of mHa1 and mHb4 around the nucleus, the endogenous keratin network collapsed and localized around the nucleus. Furthermore, the densely accumulated endogenous keratin surrounded the ring-like aggregates with partial co-localization. However, when transfected into the rat epidermal keratinocytes, mHa1 and mHb4 were able to co-localize with the well-developed cytoskeleton of endogenous keratins. These results showed that, in contrast to keratin pairs K5/K14 and K8/K18, the mHa1/mHb4 pair is unable to develop an extensive keratin network on its own, and that there are possible differential abilities among these hair keratins and other keratins to form well-developed cytoplasmic networks.
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Report
(3 results)
Research Products
(3 results)
