Molecular Biological Analysis of Wound Healing
| Project/Area Number |
07670967
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Juntendo University |
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Principal Investigator |
TSUBOI Ryoji Department of Dermatology, Juntendo University School of Medicine, Assistant Professor, 医学部, 講師 (70221421)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | wound healing / animal model / endothelin / IGF-I / IGFBP-1 / gel contraction / diabetes / povidone iodine / IGF |
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| Research Abstract |
1 The stimulatory effect of recombinant human insulin-like growth factor-I (rhIGF-I) and recombinant human insulin-like growth factor-binding protein-1 (rhIGFBP-1) on wound healing was assessed using diabetic db/db mice and normal rabbits. Full thickness wounds of 6 mm diameter were prepared on the backs of diabetic C57BL/KsJ db/db mice and on the inner sides of normal rabbit ears. Various concentrations of rhIGF-I and/or rhIGFBP-1 were applied locally to the open wounds of db/db mice once daily for 5 days and to the covered wounds of normal rabbits once after wounding. Sections of the wounds were evaluated histologically on the 7th or 8th day by measuring reepithelialization (%), area of granulation tissue (mm2) and capillary numbers. Wound repair was accelerated by each of the treatments in descending order of rhIGF-I plus rhIGFBP-1, rhIGF-I,rhIGFBP-1, and vehicle alone. In db/db mice, the combination of 50 mg rhIGF-I and 165 mg rhIGFBP-1 (equimolar ratio) significantly stimulated gr
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anulation tissue formation (p<0.01) and capillary numbers (p<0.05). (Ref 1)
2 The effect of ultraviolet B (UVB) irradiation on Endothelin-1 (ET-1) and ET receptor expressions was examined using cultured normal human keratinocytes. Keratinocytes secreted ET-1 in the medium at a level of 2.1 pg/day/105 cells. UVB irradiation up to 10 mJ/cm2 increased ET-1 secretion 3-fold, and potenitated expressions of mRNA for ET-1. Both ETA and ETB receptor mRNAs were detected in kerationocytes, and their expressions were up-regulated by 5 mJ/cm2 UVB irradiation. (Ref 2)
3 Topical application of sugar and povidone iodine (SP) on wounds was assessed using animal models. The wounds in db/db mice treated with SP displayd good repair rates compared to those for non-treated mice. The wounds in normal rabbits treated with SP had good repair rates for all three parameters compared with those for non-treated rabbits. (Ref 3)
4 Involvement of IGF-I and (IGFBP-1 in wound contraction was studied using an in vitro model. Human dermal fibroblasts (1*105 cells/ml) were incorporated into a porcine type I collagen (0.21% final) in serum-free medium. The fibroblast-embedded collagen gels in a 12-well plate were floated from the well, and various reagents were then added to the assay medium. The surface area of the gel was calculated by measuring the diameters of the collagen gel. IGF-I at high doses (30 and 100 ng/ml) revealed 6.8% and 7.7% gel contraction, respectively, and des (1-3) IGF-I at 10 ng/ml produced a 4.5% gel contraction. Meanwhile, IGFBP-1 did not induce any significant contraction. A combination of IGF-I and IGFBP-1 at 1 ng/ml of each reagent, a concentration at which gel contraction was not observed when each of the reagents was tested individually, produced a 14% gel contraction. When anti-IGF-I,anti-IGFBP-1 or anti-IGF-I receptor antibody was added to the assay medium, the IGF-I and IGFBP-1-induced gel contraction was significantly suppressed (p<0.001). (Ref 4) Less
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Report
(3 results)
Research Products
(13 results)
