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Observation of tumorangiogenesis as a fundamental experiment for novel cancer angiogenesis suppression therapy targeting

Research Project

Project/Area Number 07670979
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionTohoku University

Principal Investigator

TAKAI Yoshihiro  Tohoku School of Medicine Dept. Rad. associate Prof., 医学部・附属病院・放射線部, 助教授 (50107653)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
KeywordsTumor angiogenesis / swandwich chamber / hypoxic cell / 腫瘍血管
Research Abstract

Purpose : To investigate tumor angiogenesis using skin hold chamber with clear window. This is a basic experiment to invent novel cancer therapy targeting tumorangiogenesis suppression. Material and Method : A new skin hold mouse chamber was developed being based on "sandwich chamber" devised by Hori. This chamber was made of aluminum and hand-made. WHT/Ht mice, weighing 15-20g each, were used, and the chamber was inserted into skin pouch of the back of mouse. To observe the development of tumor tissue and tumor angiogenesis, we trasplanted a fragment of about 0.1 mm3 squamous cell carcinoma H (sccH) or fibrosarcoma YAS (fib YAS) within the chamber. The former is the most radiosensitive tumor with 16% of hypoxic fractrion, the latter is the most radioresistant tumor with 65% hypoxic fraction among 5 syngenic tumor. RESULTS : In case of sccH,tumor angiogenesis was observed from 3 days after tumor transplatation, and central necrosis was found on 6 or 7 days after transplantation. In case of fib YAS,angiogenesis was begin just 2 days after transplantation and central necrosis was hardly found. The behavior of blood of tumor vessel in sccH which was investigate by video, was different from that of fib YAS.The blood flow of sccH was continuous and that of fib YAS was intermittent. The latter is seemed to be good candidate for angiogenesis suppression thrapy.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Y.Takai: "single dose radiosensitivity of four murine solid tumors and a pvedictive assay for the curability in situ" J.Rad.Res.(1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Takai: "Two different type of cell loss pattervs of murine tumors and their covvesponding histological findings…" Strahlentherapie and Onlcalogie. 169. 317-322 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Takai: "Tumor kinetics and timing of the second dose to improve tumor radiocurability-A Prediction at the best…" Oncology. 50. 141-147 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Takai: "Two different type of cell loss patterns of murine tumors and their corresponding histological findings and possible mechanisms of production of hypxic cells." Strahlentherapie und Oncologie.169. 317-322 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Takai: "Tumor Kinetics and timing of the second dose to improve tumor radiocurability-A prediction of the best timing for fractionation by measuring ^<125>I-Iododeoxyuridine uptake in situ." Oncology. 50. 141-147 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Takai: "Single dose radiocurability of four murine solid tumors and a predictive assay for the curabilty is situ." J.Radiation Research. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y. Takai et al.: "Single dose vadiosensitivity of four murine solid tumors and a predictive assay for the curability in situ:" J. Radiation Research.(1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 有賀久哲,高井良尋 他.: "食道癌術後照射の検討" 日本放射線腫瘍学会誌. vol.8.4. 347-355 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshihiro Takai et al.: "Combination therapy with pions and schizophyllan (spg) for murine B-16 melanoma." Int. J. Oncology. 2. 813-816 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshihiro Takai et al.: "Tumor kinetics and timing of the second dose to improve tumor radiocurability prediction of best timing for fractionation by measuring 125I-IUdR uptake in situ" Oncology. 50. 141-147 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshihiro Takai et al.: "Tow different types of cell lose patterns of murine tumors and their corresponding histological findings and possible mechanisms of production of hypocix cells." Strahlenther Onckologie. 169. 317-322 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshihiro Takai et al.: "Combination therapy of single or fractionated X-rays and schizophyllan" Int. J. Oncology. 4. 385-389 (1994)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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