| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Research Abstract |
The use bone-seeking agents, such as the calcium analogue, strontium-89 chloride, or the radiolabeled phosphate compound, samarium-158 EDTMP provides effective means of dilivering systemic radiation therapy for osseous metastases. Bone marrow depression, however, remains a main side effect. Enhancement of skeletal uptake is, therefore, required. The purpose of this study is to enhance the skeletal uptake of these radiopharmaceuticals in rat models by dietary manipulation.
Eight-week-old male Wistar rats (n=18) were housed for two weeks. Three models with different bone metabolism were established by means of dietary manipulation ; normal control (C), low-calcium group (A), and low-phosphate group (B). Ca-47 chloride and Tc-99mMDP were injected at the second week and 24-h global skeletal uptake (GSU) was measured for Tc-99m and Ca-47, respectively. Rats were, then sacrificed. Each femur was weighed and counted for Ca-47. The values for 24-h GSU (%) of Tc-99m MDP were as follows ; C group : 51.7 (]SY.+-。[) 2.7, A group : 49.6 (]SY.+-。[) 3.5, B group : 69.3 (]SY.+-。[) 5.6 (p<0.01). The values for 24-h GSU (%) of Ca-47 chloride were as follows ; C group : 80.2 (]SY.+-。[) 2.7 A group : 93.7 (]SY.+-。[) 3.1 (p<0.01), B group : 72.3 (]SY.+-。[) 4.3 (p<0.01). The values for femoral uptake (% dose/g) of Ca-47 were as follows : C group : 3.76 (]SY.+-。[) 0.33, A group : 4.27 (]SY.+-。[) 0.15 (p<0.01), B group : 3.09 (]SY.+-。[) 0.18 (p<0.01). In conclusion, a 34.0% increase in GSU of Ca-47 in the low-calcium group were observed. Enhancement of skeletal uptake of Tc-99m MDP and Ca-47 chloride was archived by the dietary manipulation.
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