| Project/Area Number |
07671034
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Radiation science
|
|---|
| Research Institution | Kansai Medical University |
|---|
Principal Investigator |
AKAGI Kiyoshi KANSAI MEDICAL UNIVERSITY,FUCULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (30098115)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ITAGAKI Yasushi KANSAI MEDICAL UNIVERSITY,FUCULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 助手 (00193409)
HARIMA Keizou KANSAI MEDICAL UNIVERSITY,FUCULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (20121973)
|
|---|
| Project Period (FY) |
1995 – 1996
|
| Project Status |
Completed (Fiscal Year 1996)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | Irradiation / Experimental Tumor / Cell Cycle / アポトーシス / 放射線 / 抗Fas抗体 / 照射効果 |
|---|
| Research Abstract |
The cell cycle was analyzed using double staining with an anti-bromodeoxyuridine (BrDU) monoclonal anti-body and propidium iodide (PI). Changes in cell kinetics after irradiation were compared with those seen by the conventional PI-based DNA histogram method. The effect of irradiation on cell kinetics has been studied primarily by counting G2 arrested cells. By the present BrdU method, a rapid tradition, from G1 to S-phase was observed within 2h of irradiation, followed by G1 blook. Cell in the S-phase progressed to G2+M where they arrested, resulting in a decreased percentage of S cells, (<5%). Release of G1 after>18h. The initial Glarrest induced by irradiation was confirmed for the first time by the present BrdU-PI double atsining.
|
