| Project/Area Number |
07671037
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | KANSAI MEDICAL UNIVERSITY |
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Principal Investigator |
HARIMA Yoko Kansai Med.Univ., Radiology, Assistant Professor, 医学部, 講師 (80140276)
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| Co-Investigator(Kenkyū-buntansha) |
NAGATA Kenji Kansai Med.Univ., Radiology, Research Associate, 医学部, 助手 (30247928)
IMAMURA Masahiro Kansai Med.Univ., Radiology, Research Associate, 医学部, 助手 (40268339)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Bax / Bcl-2 / Radiotherapy / Cervical carcinoma / 癌関連遺伝子 |
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| Research Abstract |
Purpose : The ratio of Bcl-2 to Bax expression determines survival or death following an apoptotic stimulus. In order to establish a new predictor of the outcome of treatment for human cervical carcinoma, we investigated the relationship between the expressions of the Bax and Bcl-2 proteins and the response to radiotherapy after the administration of 10.8 Gy.
Methods : A total of 44 patients with histologically proven carcinoma of the uterine cervix, including three with recurrent cervical stump carcinomas, were treated with definitive radiotherapy. The presence of mutations in exons 5-8 of the p53 gene was analyzed by a single-strand conformation polymorphism analysis and DNA sequencing.
Results : Forty patients were found to have wild-type p53, and the remaining four had mutant p53. The Bax and Bcl-2 protein expressions prior to radiotherapy did not correlate with response or survival. However, the Bax and Bcl-2 protein expressions after radiotherapy correlated with both response or survival. Bax-positive tumors showed significantly better responses than the Bax-negative tumors after 10.8 Gy radiation (P=0.0002). In contrast, the Bcl-2-positive tumors showed significantly poorer responses than the Bcl-2-negative tumors after radiation (P=0.002). Increased Bax expression after the 10.8 Gy radiotherapy was found to be correlated with good survival (P=0.04). In contrast, increased Bcl-2 expression after such radiotherapy was correlated with poor survival (P=0.002).
Conclusion : The levels of Bax and Bcl-2 expression after 10.8 Gy of radiotherapy are useful prognostic markers in patients with human cervical carcinoma.
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