• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Physiological and molecular biological studies on the pathogenesis of impaired insulin secretion in diabetes mellitus.

Research Project

Project/Area Number 07671128
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

ISHIDA Hitoshi  Associate Professor, Department of Metabolism and Clinical Nutrition, Kyoto University School of Medicine, 医学研究科, 助教授 (80212893)

Co-Investigator(Kenkyū-buntansha) SEINO Yutaka  Professor, Depatment of Metabolism and Clinical Nutrition, Kyoto University Scho, 医学研究科, 教授 (40030986)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsDiabetes mellitus / Pancreatic beta-cells / Insulin secretion / Patch-clamp technique / ATP-sensitive K^+ channels / Voltage-dependent Ca^<2+> channels / Intracellular Ca^<2+> / パッチクランプ法 / 細胞内カルシウム濃度
Research Abstract

As one of the major characteristics in pathophysiological aspects in noninsulin-dependent diabetes mellitus (NIDDM), the selective impairment of glucose-induced insulin secretion has been well known. On the other hand, the insulin secretory capacity is rather enhanced in response to the stimuli other than glucose. We, therefore, investigated the activity of voltage-dependent Ca^<2+> channel (VDCC) directly using the patch-clamp technique, which has a very important role on the regulation of intracellular Ca^<2+> levels. Using single beta-cells obtained by the dispersion of pancreatic islets of GK rats with genetically NIDDM,the L-and T-types of Ca^<2+> channel currents were recorded by whole cell recording. In addition, in order to investigate the intracellular mechanisms for modulating VDCC activities throuph glucose metabolism without mediating the K_<ATP> channel closure, the perforated patch using nystatin method was also utilized. Both of L-and T-type VDCC activities were found to be significautly enhanced after the membr anedepolarization in GK beta-cells when compared to the controls. In control beta-cells, the VDCC activities were more augmented after glucose loading in perforated patches. However, such an augmentation was not to be found in GK beta-cells. These phenomena seem to be closely related to the glucose selectivity of the impairment of insulin secretory capacity found in NIDDM beta cells of GK rats.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] K. Obayashi, et al.: "Angiotensin II inhibits protein kinase A-dependent CI^- conductance in heart via PTX-sensitive G proteins." Circulation. 95 (1). 197-204 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Kato, et al.: "Alterations in basal and glucose-stimulated voltage-dependent Ca^<2+> channel activities in pancreatic β cell of non-insulin-dependent diabetes mellitus GK rats." J Clin Invest. 97 (11). 2417-2425 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A. Ishida-Takahashi, et al.: "Block of pancreatic ATP-sensitive K^+ channels and insulinotropic action by the antiarrhythmic agent cibenzoline." Br J Pharmacol. 117. 1749-1755 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N. Inagaki, et al.: "Expression and role of intotropic glutamate receptors in pancreatic islet cells." FASEB J. 9 (5). 686-691 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y. Okamoto, et al.: "Hyperresponse in calcium-induced insulin release from electrically permeabilized pancreatic islets of diabetic GK rats and its defective augmentation by blucose." Diabetologia. 38 (7). 772-778 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K. Masuda, et al.: "Effect of Troglitazone (CS-045) on insulin secretion in isolated rat pancreatic islets and HIT cells : an insulinotropic mechanism distinct from glibenclamide." Diabetologia. 38 (1). 24-30 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Obayashi, et al.: "Angiotensin II inhibits protein kinase A-dependent Cl^- conductance in heart via PTX-sensitive G proteins" Circulation. 95(1). 197-204 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kato, et al.: "Alterations in basal and glucose-stimulated voltage-dependent Ca^<2+> channel activities in pancreatic beta cell of non-insulin-dependent diabetes mellitus GK rats." J Clin Invest. 97(11). 2417-2425 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Ishida-Takahashi, et al.: "Block of pancreatic ATP-sensitive K^+ channels and insulinotropic action by the antiarrhythnic agent cibenzoline." Br J Pharmacol. 117. 1749-1755 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Inagaki, et al.: "Expression and role of ionotropic glutamate receptors in pancreatic islet cells." FASEB J. 9(5). 686-691 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Okamoto, et al.: "Hyperresponse in calcium-induced insulin release from electrically permeabilized pancreatic islets of diabetic GK rats and its defective augmentation by glucose." Diabetologia. 38(7). 772-778 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Masuda, et al.: "Effect of Troglitazone (CS-045) on insulin secretion in isolated rat pancreatic islets and HIT cells : an insulinotropic mechanism distinct from glibenclamide." Diabetologia. 38(1). 24-30 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Obayshi,et al.: "Angiotensin II inhibits protein kinase A-dependent Cl conductance in heart via PTX-sensitive G proteins." Circulation. 95(1). 197-204 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Kato,et al.: "Alterations in basal and glucose-stimulated voltage-dependent Ca^<2+> channel activities in pancreatic β cell of non-insul in-dependent diabetes mellitus GK rats." J Clin Invest. 97(11). 2417-2425 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Ishida-Takahashi,et al.: "Block of pancreatic ATP-sensitive K^+ channels and insulinotropic action by the antiarrhythmic agent cibenzoline." Br J Pharmacol. 117. 1709-1715 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] N.Inagaki,et al.: "Expression and role of ionotropic glutamate receptors in pancreatic islet cells." FASEB J. 9(5). 686-691 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Okamoto,et al.: "Hyperresponse in calcium-induced insulin release from electrically permeabilized pancreatic islets of diabetic GK rats and its defective augmentation by glucose." Diabetologia. 38(7). 772-778 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Masuda,et al.: "Effect of Troglitazone (CS-045) on insulin secretion in isolated rat pancreatic islets and HIT cells : an insulinotropic mechanism distinct from glibenclamide." Diabetologia. 38(1). 24-30 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] K. Masuda, H. Ishida et al.: "Effects of Troglitazone(CS-045) on insulin secretion in isolated rat pancreatic islets and HIT cells: an insulinotropic mechanism distinct from glibenclamide." Diabetologia. 38(1). 24-30 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y. Okamoto, H. Ishida et al.: "Hyperresponse in calcium-induced insulin release from electrically permeabilized pancreatic islets of eiabetic GK rats and its defective augmentation by glucose." Diabetologia. 38(7). 772-778 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] N. Inagaki, H. Ishida et al.: "Expression and role of ionotropic glutamate receptors in pancreatic islet cells." FASEB J. 9(5). 686-691 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Suzuki, H. Ishida et al.: "The urinary concentration of sialic acid is increased in non-insulin-dependent diabetic patients with microangiopathy: a possible useful marker for diabetic microangiopathy." Diabetic Med. 12(12). 1092-1096 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Miura, H. Ishida et al.: "Effect of chitosan on blood glucose and lipid in normal and diabetic mice." Biol Pharm Bull. 18(11). 1623-1625 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A. Ishida-Takahashi, H. Ishida et al.: "Block of pancreatic ATP-sensitive K^+ channels and insulinotropic action by the antiarrhythmic agent cibenzoline." Br J Pharmacol. (in press). (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi