| Project/Area Number |
07671145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内分泌・代謝学
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| Research Institution | Kyushu University |
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Principal Investigator |
UMEDA Fumio Faculty of Medicine KYUSHU UNIVERSITY Associate Professor, 医学部, 助教授 (80150431)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Toshihiko Faculty of Medicine KYUSHU UNIVERSITY Assistant, 医学部, 助手 (20198683)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | diabetes mellitus / microangiopathy / atherosclerosis / vascular endothelial cells / vascular smooth muscle cells / prostacyclin / prostaglandin |
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| Research Abstract |
The mechanism of PSF-induced prostacyclin (PGI_2) production by vascular endothelial cells (EC) : PSF stimulated PGI_2 as well as prostaglandin (PG) E_2 production by EC in a time- and dosedependent manner. This PSF-induced PGI_2 production did not require the nascent protein synthesis but was dependent on the extracellular Ca_<2+>. PSF increased cytosolic Ca_<2+> concentration of EC by influx from extracellular space independently of L-type Ca_<2+> channel.
Expression of PSF mRNA in the kidney of streptozotocin (STZ) -induced diabetic rats : PSF mRNA was significantly decreased in the kidney from STZ-diabetic rats as compared with the control rats (PSF/b-actin ; 0.77 (]SY.+-。[) 0.05 in diabetic rats vs.1.0 in controls).
Effect of high glucose culture on the expression of PSF mRNA in vascular smooth muscle cells (VSMC) : PSF mRNA/28S rRNA ratio was decreased (by 35%) in VSMC cultured with 500 mg/dl glucose as compared with VSMC cultured with 100 mg/dl glucose.
Localization of PSF in human tissues : Immunohistochemical analysis using a polyclonal antibody against PSF demonstrated that PSF was localized in the VSMC of the media and EC of medium to small-sized arteries. In lung tissue, PSF was localized in smooth muscle cells as well as epithelial cells of the bronchi.
Immunohistochemical study of PSF in the coronary arteries : PSF was localized in VSMC in the media of the coronary arteries. However, the level of PSF was extremely lower in the coronary arteries from the patients who died of ischemic heart disease than those from the patients without ischemic heart disease.
Immunohistochemistry of PSF in VSMC : Immunocytochemical staining of PSF was decreased in VSMC cultured with 500 mg/dl glucose as compared with those cultured with 100 mg/dl glucose.
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