• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

LIPID TRANSPORT SYSTEM IN THE LIVER

Research Project

Project/Area Number 07671155
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionTEIKYO UNIVERSITY

Principal Investigator

TERAMOTO Tamio  TEIKYO UNIVERSITY SCHOOL OF MEDICINE ASSOCIATE PROFESSOR, 医学部, 助教授 (20133077)

Co-Investigator(Kenkyū-buntansha) KINOSHITA Makoto  TEIKYO UNIVERSITY SCHOOL OF MEDICINE LECTURER, 医学部, 講師 (70186295)
MATSUKI Norio  UNIVERSITY OF TOKYO DEPARTMENT OF PHARMACY ASSOCIATE PROFESSOR, 薬学部, 助教授 (70126168)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsapoB / suncus / hepatocytes / ACAT / MTP / スンクス
Research Abstract

We have previously shown that fatty liver was easily induced in suncus by starvation and that the plasma level of apolipoprotein B (apo B) was very low. We also previously reported that a defect in the assembling process of apo B-containing lipoprotein (very low density lipoprptein, VLDL) may be one of the reasons for the low level of plasma apo B and for induction of fatty liver by starvation in suncus. We found that hepatic acyl coenzyme A cholesterol acyltransferase (ACAT) activity is almost absent in the animals, resulting in decreased cholesteryl ester conteints in the liver. A deficiency of cholesteryl ester in suncus liver may be one of the reasons for the defect in the assembling process of apo B-containing lipoproteins. Another possibility is a defect in apo B-synthesis in the liver, resulting in hypobetalipoproteinemia and in fatty liver. We investigated the hepatic syntetic rate of apo B and intrahepatic degradation of apo B using primary cultures of hepatocytes derived from … More suncus and rats, and using N-acetylleucylleucynorleucinal, an inhibitor of apo B degradation we estimated the intrahepatic degradation of apo B.In order to compare the apo B syntesis of suncus with that of rat, we estimated it as the ratio of apo B syntesis over albumin which were newly syntesized in hepatocytes. The apo B syntesis in suncus was between 14% and 29% of that in rat. Intracellular degradation of apolipoprotein B was not observed in suncus hepatocytes, while it was obvious in rat hepatocytes. This evidence suggests that the low secretion rate of apo B-lipoprotein in suncus was not resulted from enhanced intrahepatic degradation of apo B but may be from low syntetic rate of apo B in suncus. Another possibility of the defect of VLDL-assembly is low activity of microsomal triglyceride transfer protein, which has been reported to be deficient in abetalipoproteinemia. We assayd the activity in suncus liver and intestinal mucosa. While the activity in suncus intestinal mucosa was almost same as that of rat, it was about 30% of rat in the liver. When suncus were starved for 12 hours, the activity was not changed. However, when the staved animals were refed, the activity was enhanced significantly. The evidence is compatible with rapid recovery from fatty liver when the animals were refed.
In conclusion, fatty liver observed by starvation may be resulted from low ACAT activity, low apo B synthesis, and low MTP activity, suggesting that these factors play a key role in VLDL-assembling. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Nagayoshi,A.et al: "Defect in assembling process of very-low density lipoprotein in suncus liver." J.Biochem.117. 787-793 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagayoshi,A.et al: "Role of acylcoenzyme A chdesterol acyltransferase in intrahepatic processing …" J.Biochem.118. 259-264 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Teramoto,T.et al: "Binding of ritamin D to Low-Density-Lipoprotein(LDL) and LDL Receptor …" Biochem.Biophys.Res.Commun.215. 199-204 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kinoshita,M.et al: "CETP is determinant of serum LDL-chdesterol but not HDL-chdesterol in healthy …" Atherosclerosis. 120. 75-82 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Teramoto,T.et al: "Characterization of low-density-lipoprotein in apolipoprotein E deficiency in a …" Clinica.Chimica.Acta.247. 159-166 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Teramoto,T.et al: "Dose-depondent effect of niceritrol on plasma lipoprotein-α." Scand J Clin Lad Invest. 56. 359-365 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagayoshi, A., Matuki, N., Saito, H., Tsukamoto, K., Kaneko, K., Wakashima M., Kinoshita, M., Yamanaka, M., and Teramoto, T: "Defect in assembling provess of very-low-density lipoprotein in suncus liver." J.Biochem. 117. 787-793 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagayoshi, A., Matsuki, N., Saito, H., Kaneko, K., Shimazu, N., Suga, S., Wakashima, M., Kinoshita, M., Ymanaka, M., and Teramoto, T: "Role fo acylcoenzyme A cholesterol acyltransferase in intrahepatic processing of spo B-Lipoprotein in suncus." J.Biochem. 118. 259-264 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sato, T., Fukasawa, M., Kinoshita, M., Arai, H., Saeki, T., Naraki, T., Iwasaki, Y., Teramoto, T., Takahasi, K., Saito, Y., Inoue, K: "Enzyme-linked immunosorbent assay for cholesteryl ester transfer protein in human serum." Clinica Chimica Acta. 240. 1-9 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Teramoto, T., Endo, K., Ikeda, K., Kubodera, N., Kinoshita, M., Yamanaka, M., and Ogata, E: "Binding of vitamin D to Low-Density-Lipoprotein (LDL) and LDL Receptor-Mediated Pathway into Cells." Biochem.Biophys.Res.Commun. 215. 199-204 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kinoshita, M., Teramoto, T., Simazu, N., Kaneko, K., Ohta, M., Koike, T., Hosogaya.S., Ozaki, Y., Kume, S., Yamanaka, M: "CETP is determinant of serum LDL-cholesterol but not HDL-cholesterol in healthy Japanese." Atherosclerosis. 120. 75-82 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Teramoto, T., Kinoshita, M., Kawamura, M., Yi-Qiang Liang.Kaneko, K., Shimazu, N., Suga.S., Akatuka, N.Yamanaka, M: "Characterization of low-density-lipoprotein in apolipoprotein E deficiency in a patient without coronary atherosclerosis." Clinica Chimica Acta. 247. 159-166 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Teramoto, N.Yamada, H.Shimano, Y.Oka, H.Itakura, Y.Saito, N.Morisaki, K.Sirai, T.Ishikawa, N.Tada, H.Ito, T.Yamanouchi, T.Matsushima, M.Kawakami, T.Murase, M.Okubo, Y.Totsuka, M.Kikuchi: "Dose-dependent effect of niceritrol on plasma lipoprotein-a." Scand J Clin Lab Invest. 56. 359-365 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hashimoto, Y., Tsukamoto, K., Teramoto, T., Kurokawa, K., Watanabe, T: "Purification and chareterization of low density lipoprotein containing apolipoprotein B-48 from the plasma of an apo E-deficient patient." Clin Chim Acta. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagayoshi,A et al: "Defect in assembling process of very-low-density lipoprotein in suncusliver" J.Biochem. 117. 787-793 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagayoshi,A et al: "Role of acylcoenzyme A cholesterd acyltrans ferase in intrahepatic...." J.Biochem. 118. 259-264 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Teramoto,T.et al: "Binding of vitamin D to Low-Density-Lipoprotein (LDL) and LDL Receptor...." Biochem.Biophys.Res.Commun.215. 199-204 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kinoshita,M.et al: "CETP is determinant of serum LDL-cholesterol but not HDL-chdesterd...." Atherosclerosis. 120. 75-82 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Teramoto,T.et al: "Characterization of low-density-lipoprotein in apolipoprotein E defieiency...." Clinica.Chimica.Acta.247. 159-166 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Teramoto,T.et al: "Dose-dependent effect of niceritorol on plasma lipoprotein-α." Scand J Clin Lab Inrest. 56. 359-365 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagayoshi A, Teramoto T: "Defect in assimbly process Very-Low-Density lipgnctein in Simues liver An arumol model of fatty eiver" J. Biochem. 117. 787-793 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nagayoshi A, Teramoto T: "Role of acogl CoA choles tesol achlhansfcrasc in introhepatic procvssing of ape β-lipoprcteen in Seeneccs" J. Biochem. 118. 259-264 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato T, Teramoto T: "Enzyme linked immuncsortant assay for cholosfeyl esfer henotion protein in human serum" Clin Chin Acta. 240. 1-9 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Teramoto T et al.: "Clinical efficauy of Fluuastatin for hyperligidenua in Japanese patients" Am. J. Cardiol. 76. 33A-36A (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Teramoto T. et al.: "Binding of Vitamin D to Low Perdity Lipoprden(LDL) and LDL receptor mediated pathway into cells" Biochrim. Biopliys. Res. Commun. 215. 199-204 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kinoshita M., Teramoto T.: "CETP is a deferminant of serum LDL Cholen of out not HDL cholesferol in hrealtly Japanese" Athcrosclerosis. 120. 75-82 (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi