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Cloning of cDNAs encoding multidrug-resistance (MDR) associated, P-glycoprotein related protein (mini-P-glycoprotein) overexpressed in murine MDR cells.

Research Project

Project/Area Number 07671193
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionMie University

Principal Investigator

KAWAI Kazuo  Mie University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (60152899)

Co-Investigator(Kenkyū-buntansha) KUSANO Itsuo  Mie University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (90126970)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsCancer Chemotherapy / Multidrug Resistance / Multiderug Resistance Associated Protein / P-glycoprotein / 化学療法 / 抗癌剤 / 遺伝子クローニング / 単クローン抗体
Research Abstract

As reported previously, an approximately 65 kDa cellular protein, designated mini-P-glycoprotein (Pgp_<mini>), was originally identified in multidrug resistant (MDR) murine cell lines. In those studies, the Pgp_<mini> was suggested to share both an antigen epitope and some nucleotide sequences with P-glycoprotein (P-gp), and increased expression of this protein was found to be correlated with the level of MDR,although, in contrast to the P-gp, Pgp_<mini> was neither glycosylated nor phosphorylated. In the present study, we also demonstrated that some human MDR cells overexpress Pgp_<mini> as well as P-gp. Furthermore, we constructed a cDNA library from a highly MDR murine cell line into lambdagt10, and using a modified differential hybridization screening, we have identified cDNA clones which encode Pgp_<mini>. After the subcloning into plasmid vectors, we sequenced them. Sequence homology search of this gene shows it to be most closely related to P-gp, particularly mdrla. To clarify the function, works are currently underway to clone hybridomas producing monoclonal antibodies specific to Pgp_<mini> and to transfect with the cDNA into drug-sensitive cells to express it.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (1 results)

All Other

All Publications (1 results)

  • [Publications] K.Kawai: "Identification of a Prglycepnotein related protein(mini-P-glycaprsfein)which is ounexpeped in multdray relifant old" Biochemical and Bioplyoical Reseauh Communication. 198. 804-810 (1994)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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