| Project/Area Number |
07671261
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Juntendo University |
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Principal Investigator |
SUZUKI Sigenobu JUNTENDO UNIV,ASSISTANT PROFESSOR, 医学部, 講師 (20271281)
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| Co-Investigator(Kenkyū-buntansha) |
OSADA Siori JUNTENDO UNIV,RESEARCH ASSOCIATE, 医学部, 助手 (50296860)
SETOGUTHI Yasuhiro JUNTENDO UNIV,RESEARCH ASSOCIATE, 医学部, 助手 (90206649)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | erythropoietin / renal anemia / mesothelial all / polycystic lindney / gene thrapy / adens virus retor / 多発性のちほう腎 / DBA / 2FG-pcyマウス / 遺伝子導入 / 腹膜中皮細胞 / 慢性腎不全 / アデノウィルス / 多発性のうほう腎 / 2FG-pcy |
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| Research Abstract |
Human erythropoietin (HuEPO) gene transfer to mesothelial cells was performed in vitro and in vivo. Recombinant replication-deficient adenoviruss containing HuEPO cDNA (ASCMVEPO), E.coli lacZ gene (AdCMVlacZ), or an endogenous gene driven by the cytomegalovirus promotor/enhancer were constructed. In vitro studies demonstrated that mesothelial cells secreted HuEPO synthesized by the innoculation of AdCMVEPO in a polarized fashion. The sialylated oligosaccharides associated with the HuEPO from AdCMVEPO-treated mesothelial cells occupied 78 +/- 0.7% of the total oligosaccharide pool. For in vivo study, the DBA/2FG-pcy mouse, a model for human autosomal recessive polycystic kindney disease with progressive anemia, was used. A single intraperioneal administration of AdCMVEPO induced HuEPO synthesis in the peritoneum and a marked increase in erythrocyte production. The maximal increase (18 +/- 2%) in hematocrit was observed on day 28, and it remained elevated for 40 days. These results show that intraperitoneal administration of AdCMVEPO improves renal anemia appropriately in DBA/2FG-pcy mice.
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