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Roles of Wnt-1 protein in developing central nervous system in rat embryos

Research Project

Project/Area Number 07671282
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Embryonic/Neonatal medicine
Research InstitutionInstitute for Developmental Research, Aichi Human Service Center

Principal Investigator

MATSUDA Motoko  Institute for Developmental Research, Aichi Human Service Center, Senior Researcher, 発生学部, 主任研究員 (40165832)

Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
KeywordsRat embryo / Central nervous system / Whole embryo culture / Wnt-1 / Yolk sac / 全胚培養 / 卵黄のう / 胚培養 / 脳神経系 / 形態形成 / 神経管形成 / ペプチド抗体
Research Abstract

Rat embryos (day 9.5 of gestation) cultured for 48 hours in medium supplemented with anti-Wnt-1 antibodies (35-50mug/ml), which were raised in rabbits against Wnt-1 polypeptide (aa.241-256), failed to develope the ventral part of the neural tube. In some regions of the neural tube, the ventral part was deleted and in other regions of the neural tube neuroepithelial cells showed an irregular sha ; e and accumulated in the ependymal layr. Some of the treated embryos developed outpockeings similar optic vesicles at the level of the heart. The antibodies added in the medium penetrated to the ependymal layr in the ventral part of the neural tube. When the embryos were treated with a low dose of the antibody (1 mug/ml), angiogenesis of the yolk sac was inhibited. The size of the treated and the control embryos. This phenomenon was also oserved in embryos cultured in the medium supplemented with antisenseorigonucleotide (10^<-2>muM) against wnt-1. embryos cultured in the medium supplemented with anti-b-FGF antibody and anti-FGF-5 antibody developed normally.
These results suggest that Wnt-1 protein plays roles in cell proliferation and cell morphogenesis in the ventral part of the neural tube, resulting in morphogenesis of the neural tube. Wnt-1 may also function in angiogenesis of yolk sac by the different way from FGF.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Matsuda, M.et al.: "An open cephalic neural tube reproducibly induced by cytochalasin D in rat embryos in vitro." Zool.Sci.11. 547-553 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsuda, M et al.: "Axial rotation in rat embryos:involvement of changes in the shapes and arrangement of cells." Cell Struc.and Func.20. 1-12 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Katoh-Semba.R et al.: "Chondroitin sulphate proteoglycans in the rat brain:candidates for axon barrieres of sensory neurons and the possible modification by laminin of their actions." Eur.J.Neurosci.7. 613-621 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsuda, M.et al.: "A possible role of the nestin protein in the developing central nervous system in rat embryos." Brain Res.723. 177-189 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsuda, M.and Keino, H.: "An open cephalic neural tube reproducibly induced by cytochalasin D in rat embryos in vitro." Zool.Sci.11. 547-553 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsuda, M.and Yasutomi, M.: "Axial rotation in rat embryos : involvement of changes in the shapes and arrangement of cells." Cell Struc.and Func.20. 1-12 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Katoh-Semba, R., Matsuda, M., Kato, K.and Oohira, A.: "Chondroitin sulphate proteoglycans in the rat brain : candidates for axon barrieres of sensory neurons and the possible modification by laminin of their actions." Eur.J.Neurosci.7. 613-621 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsuda, M.and Katoh-semba, R., Kitani, H.and Tomooka, Y.: "A possible role of the nestin protein in the developing central nervous system in rat embryos." Brain Res.723. 177-189 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsuda,M.et al.: "An open cephalic neural tube reproducibly induced by cytochalasin D in rat embryos in vitro." Zool.Sci.11. 547-553 (1994)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsuda,M.et al.: "Axial rotation in rat embryos : involvement of changes in the shapes and arrangement of cells." Cell Struc.and Func.20. 1-12 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Katoh-Semba,R.et al.: "Chondroitin sulphate proteoglycans in the rat brain : candidates for axon barrieres of sensory neurons and the possible modification by laminin of their actions." Eur.J.Neurosci.7. 613-621 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsuda,M.et al.: "A possible role of the nestin protein in the developing central nervous system in rat embryos." Brain Res.723. 177-189 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsuda and Yasutomi: "Axial rotation in rat embryos : involvement of changes in the shapes and arrangement of cells." Cell Struc.Func.20. 1-20 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] K-Semba,Matsuda,et al.: "Chondroitin sulphate proteoglycans in the rat brain : candidates for axon barriers of sensory neurons and the possible modification by laminin of their actions." Eur.J.Neurosci.7. 613-621 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuda,K-Semba,et al.: "A possible role of the nestin protein in the developing central nervous system in rat embryos." Brain Res.723. 177-189 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] R. Katoh-Semba et al.: "Chondroitin sulphate proteoglycans in the rat brain: Candidates for axon barriers of sensory neurons and the possible modification by laminin of their actions" European Journal of Neuroscience. 7. 613-621 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M. Matsuda et al.: "A possible role of the nestin protein in the developing central nervous system in rat embryos" Brain Research. in press. (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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