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Bone formation by the implantation of bone marrow cells

Research Project

Project/Area Number 07671283
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionHokkaido University

Principal Investigator

MIZUNO Morimichi  Hokkaido Univ., Sch.of Dent., Inst., 歯学部, 助手 (10125354)

Co-Investigator(Kenkyū-buntansha) KUBOKI Yoshinori  Hokkaido Univ., Sch.of Dent., Prof., 歯学部, 教授 (00014001)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsOsteoblasts / Bone / Bone marrow cells / Collagen gel
Research Abstract

In this study, we attempted to form a mass of bone by the implantation of osteoblastic cells differentiated from bone marrow stromal cells. Dexamethasone or vitamin C have been utilized to induce the osteoblastic differentiation so far, however, there is no report that these cells formed bone in vivo. Recently we found that collagen gel induced the osteoblastic differentiation without dexamethasone or vitamin C.These cells with collagen gel induced bone and a volume of bone increased time-dependently in vivo. On the other hand, un-differentiated bone marrow cells with collagen gel did not form bone. These results indicate that bone marrow stromal cells which expressed the osteoblastic phenotypes could form bone effectively in vivo. The effect of collagen gel is specific for type I,and type II-V collagen did no? possess the inducing activity. Furthermore, TGF-beta enhanced the osteoblastic differentiation by collagen gel. Our results demonstrate that bone marrow cells could form a mass of bone by our procedure. The benefit of our method is 1) we do not use dexamethasone, which has a potent side-effect, 2) we utilized collagen, which shows affinity to tissues.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Mizuno M.: "Osteogenesis by Bone Marrow Stomal Cells Mainbained on Type I Collagen Matrix Gels In Vivo" Bone. 20. 101-107 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mizuno M.: "The osteoblastic MC3T3-E1 cells synthesized C-terminal propeptide of type I collagen, which promoted cell-atbachment of osfeoblasi" Biochenica et Biophysica Acba. 1310. 97-102 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mizuno M.: "Bone chondroad herin promobes allachment of osbeoblastic cells to solid-stabe subsbrates and shows affiniby to collagen" Calcified Tissne Inbernabional. 59. 163-167 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mizuno, M., Kitajima, T., Tomita, M., Kuboki, Y.: "The osteoblastic MC3T3-E1 cells synthesized C-terminal propeptide of type I collagen, which promoted cell-attachment of osteoblasts." Biochemica et Biochysica acta. 1310. 97-102 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mizuno, M., Fujisawa, F., Kuboki, Y.: "Bone chondroadherin promotes attachment of osteoblastic cells to solid-state substrates and affinity to collagen." Calcified Tissue International. 59. 163-167 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mizuno, M., Shindo, M., Kobayashi, D., Tsuruga, E., Amemiya, A., Kuboki, Y.: "Osteogenesis by bone marrow stromal cells maintained on type I collagen matrix gels" Bone. 20. 101-107 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mizuno M.: "The osteoblastic MC3T3-EI cells synthesized C-terminal propeptide of type T collagen, which promoted cell-attachment of ostesblasts" Biochemica et Biophysica Acta. 1310. 97-102 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mizuno M: "Bone Chondroad herin Promotes Attacoment of Osteoblastic Cells to Solid-State Substrates and Shows Affinity to Collagen" Calcified Tissue Inbernabional. 59. 163-167 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mizuno M.: "The osteoblaobic MC3T3-E1 cellssynthesized C-berminal propaptide of type I Cellayen,which promoted all-attachment of osteohlasts," Biochemica et Biophysica Acta. (in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mizuno M.: "TGFβ accelerated the osteogenic differertiation of bone marrow cells induced by collager matrix" Biochem.Biophys. Res.Commun.211. 1091-1098 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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