| Project/Area Number |
07671315
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
BABA Kinya MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,LECTURER, 生体防御医学研究所, 助手 (30271117)
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| Co-Investigator(Kenkyū-buntansha) |
MIMORI Kohshi MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,RESIDENT, 生体防御医学研究所, 医員
MORI Masaki MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,ASSOCIATE PROFESSOR, 生体防御医学研究所, 助教授 (70190999)
渋田 健二 九州大学, 生体防御医学研究所, 助手 (70253531)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | esophageal carcinoma / invasion / metastasis / gene / proteinase / matrix metalloproteinase / integrin / messenger RNA / cDNA サブトラクション / differential display / matrix metalloproteinase / integrin |
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| Research Abstract |
1.Study of matrix metalloproteinase 7 (MMP7)
1) We performed in vitro invasion assay using squamous carcinoma cell line KY150 and its transfectant with MMP cDNA.The expression of MMP7 in parent KY150 cell was very weak. On the other hand, its expression in transfectant was increasing and determined by Northern blot analysis. The invasion assay demonstrated that the invading cells were much frequently seen in transfectants than in parent cells (56% vs 10%).
2) To determine the effect of anti-MMP7 antibody, we added the antibody into the culture medium. Consequently the invading ability of KY150 transfectants was dramatically inhibited by the antibody (56%*16%).
These findings support the hypothesis that MMP7 is one of the major proteinases which influence the invading ability of esophageal squamous cell carcinomas.
2.Study of integrin alpha6
The expression of integrin alpha6 was dramatically increased in tumor tissues rather than normal tissues of the esophagus. This was determined by Northern blot analysis and immunohistochemical analysis. The tumors showing high degree of integrin alpha6 expression were those having deeper invasion and worse prognosis. we are studying the expression level of integrin beta1 and beta4. We are also studying the correlation between integrin expression and matrix metalloproteinase (MMP7) expression because some kind of MMPs may be regulated by signal transduction relating to the integrin.
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