| Project/Area Number |
07671334
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Tokai University |
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Principal Investigator |
INOKUCHI Sadaki School of Medicine, Tokai University Associate Professor, 医学部, 助教授 (60160008)
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| Co-Investigator(Kenkyū-buntansha) |
ANDO Kiyoshi School of Medicine, Tokai University Assistant Professor, 医学部, 講師 (70176014)
SAKAI Hideto School of Medicine, Tokai University Professor, 医学部, 教授 (80102846)
SHIMAMURA Kazuo School of Medicine, Tokai University Associate Professor, 医学部, 助教授 (00119679)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | human keratinocytes / diabetes / insulin / gene therapy / immunodeficient mice / cell transplantation / adenovirus vector / SCIDマウス |
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| Research Abstract |
We proposed to produce artificial endocrine organ composed ofcultured human skin cells into which a human hormone gene was introduced.
Human preproinsulin C-DNA driven under the methallothionein, MTT,gene promoter was cloned into a adenovirus vector. After infection of the virus, rAdMTTins, cultured human keratinocytes produced a significant amount of proinsulin in vitro. When the virus infected cells were transplanted into immunodeficient mice, balb/c nu/scid, decreased blood sugar concentration was observed for 3 days. During the same period, plasma proinsulin concentration of the mice was significantly elevated.
These results indicate that the virus infected keratinocytes transiently secrete bioactive proinsulin into blood stream of the immunodeficient mice. For stable expression of the introduced gene, we are improving a retrovirus vector for gene transfar into human keratinocytes.
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