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Prevention of hyperacute xenograft rejection by polyclonal antibodies

Research Project

Project/Area Number 07671339
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionTokyo Women's Medical College

Principal Investigator

HAYASAKA Yutaro  Tokyo Women's Medical College, Dept.of Surgery, Lecture, 医学部, 講師 (30120033)

Co-Investigator(Kenkyū-buntansha) SHINKAI Mahito  Tokyo Women's Medical College, Dept.of Surgery, Assistant, 医学部, 助手 (10216210)
FUJITA Shougo  Tokyo Women's Medical College, Dept.of Surgery, Assistant, 医学部, 助手 (58257020)
小山 一郎  東京女子医科大学, 医学部, 助手 (10266760)
船越 陽一  東京女子医科大学, 医学部, 助手 (20238653)
藤田 省吾  東京女子医科大学, 医学部, 助手 (70209055)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsPrevention / hyperacute / xenograft rejection / polyclonal antibodies / 異種抗体 / 生着延長 / 既存抗体(含,自然抗体) / 結合阻害作用 / “マスキング"作用 / _<ウサギ>抗モルモット赤血球 / 異種discordant心移植 / 異種ドナー / 術前処理 / ドナー抗原 / 抗ドナー赤血球 / マスキング作用
Research Abstract

Prevention of hyperacute xenograft rejection by polyclonal antibodies against donor luymphocytes and red cells.
T.Murakami, S.Hujita, Y.Hayasaka, M.Shinkai, Y.Funakoshi, T.Kawai, S.Fuchinoue, S.Teraoka, T.Agishi, and K.Ota.
Dept.of Surgery, Kidney Center, Tokyo Womens Medical College, Tokyo, Japan.
Intravenous administration of immunoglobulin (IVIG) at a high dosage has been reported to delay an initiation of hyperacute rejection (HAR) of the discordant xeno-grafts. In order to investigate possibility of preventing HAR of guinea pig hearts grafted to Lewis rats by IVIG,specific polyclonal antibodies were produced by immunizing rabbits against guinea pig lymphocytes and red cells (anti-GP-pAb)
Immediately before transplantation, the Lewis rats intravenously received either 1 ml of 20% human albumin as a control or 1.5-30mg/kg of the antibodies. Contraction time of the vascualized cardiac xenografts was extended from a mean of 12.2 minutes to longer than 7 hours intimately depending upon the doses of the antibodies administered (r=0.958). Immunopathological studing showed diffuse deposition of C3 and fibrinogen with a small amount of vascular deposits of rat IgM in the rejected grafts. In the functioning grafts treated with the antibodies, rabbit IgG deposition along endothelium was observed early after transplantation and becam more extensive with toime, while deposits of C3, fibrinogen and rat IgM were minimal. In conclusion, our data suggest that the rabbit antiGP-pAb. at a high dosage prevent the binding of the natural antibodies to the graft endothelium and modulate the complement activation.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] S.Fujita,et al.: "Prevention of hyperacute xenograft rejection by polyclonal antibodies against donor blood cells." Transplantation Proceedong. 28(2). 726-727 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 早坂勇太郎,他: "Long Term-DSTによる抗ドナー異種既存(赤血球凝集)抗体の抑制に関する検討" 日本移植学会雑誌『移植』(総会臨時号). Vol.30. 309- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 藤田省吾,他: "抗異種血球ポリクローナル抗体による異種移植心の生着延長効果" 日本臓器保存生物医学会. Vol.3(1). 57-66 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Fujita, et al.: "Prevention of hyperacute xenograft rejection by polyclonal antibodies against donor blood cells." Transplantation Proceedong. 28 (2). 726-727 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Hayasaka, et al.: "Prevention of anti-donor Xenopreformed antibodies with Long Term-DST" 「Transplantation」 Japanese Transplantation Association.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Fujita, et al.: "Prevention of hyperacute xenograft rejection by polyclonal antibodies against donor blood cells." Organ Biology. 3 (1). 57-66 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Fujita,et al.: "Prevention of hyperacute xenograft rejection by polyclonal antibodies against donor blood cells." Transplantation Proceedong. 28(2). 726 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 早坂勇太郎,他: "Long Term-DSTによる抗ドナー異種既存(赤血球凝集)抗体の抑制に関する検討." 日本移植学会雑誌『移植』(総会臨時号). Vol.30. 309 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 藤田 省吾,他: "抗異種血球ポリクローナル抗体による異種移植心の生着延長効果に関する研究" 日本臓器保存生物医学会. Vol.3(1). 57-66 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 藤田省吾: "抗異種赤血球/白血球ポリクローナル抗体による生着延長効果" 日本移植学会雑誌(総会臨時号). 30. 137 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 早坂勇太郎: "Long-Term DSTによる抗ドナー異種既存抗体の抑制の検討" 日本移植学会雑誌(総会臨時号). 30. 309 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] S. Fujita: "prevention of hyperacute xenograft rejection by polyclonal antibodies" The third international longress for Xenotransplantation(Abstracts). Po-4 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Touru Murakami: "prevention of hyperacute Xenograft rejection by polyclonal antibodies" INFA and WAITS 1995 in Sappro Abs.99 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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