| Project/Area Number |
07671340
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
TOMIZAWA Yasuko Tokyo Women's Medical College, Department of Cardiovascular Surg, Instructor, 医学部, 助手 (00159047)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | angiogenesis / vein / vascular prostheses / cytokine / bFGF / antithrombogenicity / endothelial cell / blood vessel prostheses |
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| Research Abstract |
Basic fibroblast growth factor (bFGF) is one of the strongest angiogenic agent, and affects all cell types involved in would healing. The distribution of bFGF is widespread, because it plays an important role in regulating cell proliferation and mitosis. Angiogenesis by bFGF is important for endothelialization in vascullar prostheses and is significant fro mshortly after implantation. Lack of angiogenic factors in the vascular graft wall may cause for unhealing neointima. Antithrombogenicity is important when the speed of blood is slow in the venous position for satisfactory patency. Natural and permanent antithrombogenicity due to host endothelial cells should be excellent for long-term patency. Accerelated endothelialization on the lumen of vascular prosthesis is important from shortly after implantation. We hypothesized that tissue-fragmented (TF-) grafts are rich of angiogenic activity from shortly after implantation and it ability may work for endothelialization. We developed a TF-graft and observed the healing process including angiogenesis and endothelialozation. Proliferation and mitosis of endothelial cells ware active in the graft wall in the arterial position 5 days after implantation. The full thickness of the intima was organized at 7 days. These results demonstrated that endogenous bFGF is important for endothelialization due to angiogenesis in fabric vascular prostheses, whereas thrombus and infection might have a negative effect. It might be possible to have patent grafts in the venous position, if we could produce stronger antithrombogenicity for a week.
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