Selective Cell Fusion Method by LASER Device
| Project/Area Number |
07671356
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
KATOH Hirotaka Tohoku University, Second Department of Surgery, School of Medicine, assistant professor, 医学部・附属病院, 助手 (00240656)
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| Co-Investigator(Kenkyū-buntansha) |
OHKOHCHI Nobuhiro Tohoku University, Second Department of Surgery, School of Medicine, assistant p, 医学部, 講師 (40213673)
DOI Hideyuki Tohoku University, Second Department of Surgery, School of Medicine, assistant p, 医学部・附属病院, 助手 (90188839)
SATOH Shunichi Tohoku University, Department of Technology, associate professor, 素材工学研究所, 助教授 (30162431)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
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| Keywords | micro-processing / cell fusion / myeloma cells (SP2) / Ouchterlony method / hybridoma / ハイブリドーマ / レーザー / SP2 / レーザートラッピング |
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| Research Abstract |
We developed a new cell fusion method which was sterile, noncontact and selective technique under the microscope, using the micro-processing device by LASER.By this technique, we succeeded in fusing myeloma cells (SP2) and lymphocyte in mouse. We also defined the proliferation of the fused cells in HAT medium and the function of the fused cells in the Ouchterlony method, i.e. production of IgG.This method is applicable to fuse cells which are difficult to be fused by conventional methods.
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Report
(3 results)
Research Products
(6 results)
