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Studies of pathogenesis on remnant liver dysfunction after extended hepatectomy, from the view points of changes in nitric oxide and adhesion molecule expression.

Research Project

Project/Area Number 07671383
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionMIE University

Principal Investigator

NOGUCHI Takashi  Mie University, College of Medical Sciencies, Professor, 医療技術短期大学部, 教授 (40144258)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordshepatic resectability / remnant liver dysfunction / endothelial cells of the hepatic sinnsoid / adhesion molecule / nitric oxide / splenectomy / splenic macrophages / microcirculation of the liver / Critical condition / 肝類洞内皮細胞機能 / critical condition / 肝類洞壁細胞機能 / 内皮細胞機能 / 接着分子発現 / 肝微小循環障害 / サイトカイン
Research Abstract

[Purpose] This study was performed to elucidate the pathophysiology of the remnant liver dysfunction after extended hepatectomy over functional resectability of the liver, based on the relationships to nitric oxide (NO) changes and adhesion molecule expression.
[Materials & Methods] Experimental adult mongorel dogs into three groups, 70%-hepatectomy (Group I), 84%-hepatectomy (Group II) and 84%-hepatectomy with simultaneous splenectomy (Group III), then serially measured following parameters until 24 hours postoperatively : 1.hepatic function test in the serum- (1) hepatocyte (GOT,GPT,AKBR), (2) endothelial cell of hepatic sinusoid (hyaluronic acid ; HA), (3) Kupffer cell (endotoxin), 2.hepatic tissue blood flow by Laser Doppler, 3.Liver tissue NO level (NO-Hb) by near-infrared spectrophotometer, and 5.ICAM-1expression in the liver tissue (immuno-histochemical staining).
[Results] The hepatocyte functions were better maintained immediatly postoperatively in Group I than in Group II.HA le … More vels as endothelial function, on the other hand, were both significantly higher beginning immediatly postoperatively and negatively correlated with liver tissue blood flow. Immediately after operation, liver tissue NO levels were significantly higher in both groups, but the changes occurred at lower levels in Group II than Group I.Expression of ICAM-1 in remnant liver tissue was observed in Group II,and was particularly high in the animals with severe liver dysfunction with high HA levels and reduced NO values. In Group III,however, endothelial cell function, Kupffer cell function and liver tissue blood flow were maintained with close to levels of Group I,resulting in good remnant hepatocyte function. Therefore, NO levels and ICAM-1 expression were significantly improved by splenectomy compared with those of Group II.
[Conclusion] Liver tissue NO varied depending on the sinusoidal endotherial cell function and liver tissue blood flow. The degree of ICAM-1 expression and the changes of NO levels reflected on the remnant liver dysfunction after extended hepatectomy in the early postoperative period. On the other hand, both the changes in NO levels and ICAM-1 expression were significantly improved by combined resection of spleen, suggesting that the spleen are involved in the remnant liver dysfunction in the early postoperative period, particularly in the progression of sinusoidal endothelial cell damage. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 野口 孝: "肝外科における肝機能の評価(1)" 外科. 57 (1). 65-77 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 野口 孝: "肝外科における肝機能の評価(2)" 外科. 57 (2). 189-200 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 川原田 嘉文: "肝後区域切除術" 消化器外科. 18 (7). 1082-1090 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Higashiguchi Takashi: "The preoperative nutritional assesment of surgical patients with hepatic dysfunction." Surgery Today Jpn. J. Surg. 25. 113-118 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 野口 孝: "虚血肝障害における類洞壁細胞機能の可逆性とその評価 -実験的・臨床的検討-" 外科. 57 (10). 1213-1216 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 野口 孝: "肉芽性肝膿瘍" 臨床消化器内科. 11 (13). 1987-1994 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 野口 孝: "領域別症候群シリーズNo.9" 日本臨床社, 4 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takashi NOGUCHI: "Estimation of hepatic function in liver surgery (1)." Surgery (in Japanese). 57 (1). 65-77 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takashi NOGUCHI: "Estimation of hepatic function in liver surgery (2)." Surgery (in Japanese). 57 (2). 189-200 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshifumi KAWARADA: "Posterior segmentectomy of the liver." Castroenterology (in Japanese). 18 (7). 1082-1090 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takashi HIGASHIGUCHI: "The preoperative nutritional assesment of surgical patients with hepatic dysfunction." Surgery Today Jpn.J.Jap.25. 113-118 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takashi NOGUCHI: "Functional reversibility and its evaluation of sinusoidal endothelial cells in ischemic liver injury-experimental and clinical studies-." Surgery (in Japanese). 57 (10). 1213-1216 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takashi NOGUCHI: "Granulomatous liver abscess." Internal medicine of clinical gastroenterology (in Japanese). 11 (13). 1987-1994 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 野口 孝: "肝外科における肝機能の評価(1)" 外科. 57(1). 65-77 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 野口 孝: "肝外科における肝機能の評価(2)" 外科. 57(2). 189-200 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 川原田 嘉文: "肝後区域切除術" 消化器外科. 18(7). 1082-1090 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Higashiguchi Takashi: "The preoperative nutritional assesment of surgical patients with hepatic dysfunction." Surgery Today Jpn J Surg. 25. 113-118 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 野口 孝: "虚血肝障害における類洞壁細胞機能の可逆性とその評価-実験的・臨床的検討-" 外科. 57(10). 1213-1216 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 野口 孝: "肉芽性肝膿瘍" 臨床消化器内科. 11(13). 1987-1994 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 野口 孝: "領域別症候群シリーズNo.9" 日本臨床社, 4 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 東口高志: "肝・腸管・骨格筋の蛋白・アミノ酸代謝からみた硬変肝切除後の肝再生促進と代謝栄養管理" 肝臓. 36(2). 133-135 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 野口孝: "肝外科における肝機能の評価(1)" 外科. 57(1). 65-77 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 嶌原康行: "肝外科における肝機能の評価(2)" 外科. 57(2). 189-200 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 野口孝: "虚血性肝障害における類洞壁細胞機能の可逆性とその評価" 外科. 57(10). 1213-1216 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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