Analysis of reguiatory mechanism of lymphocyte homing and cytokine involvement
| Project/Area Number |
07671394
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Okayama University |
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Principal Investigator |
IWAGAKI Hiromi Okayama University Hospital attached to Medical School, Department of Surgery, Assistant professor, 医学部・附属病院, 助手 (50240867)
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| Co-Investigator(Kenkyū-buntansha) |
KIKUTA Akio Okayama University Medical School, , Department of Anatomy, Lectureer, 医学部, 講師 (10116452)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Homing / CD34 / GlyCAM-1 / L-selectin / HEV |
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| Research Abstract |
GlyCAM-1 (glycosylation -dependent cell adhesion molecule-1) is one of the sialomucin-like ligands for L-selectin, which is a member of the selectin family and mediates initial adhesion of leukocytes to specialized high endothelial venules in lymph nodes and venules at sites of inflammation. GlyCAM-1, lacking a transmembrane domain, is supposed to be secreted into the blood. To understand the functional role of secreted GlyCAM-1, we performed sandwich enzyme-linked immunosoebent assay to measure GlyCAM-1 plama levels after inflammatory stimulus. BALB/c mice were injected with complete Freund"s adjuvant (CFA) in the hind footpads ; serum levels of GlyCAM-1 and L-selectin bound to GlyCAM-1 and several inflammatory cytokines, including interleukin-6 (IL-6), were measured at various intervals. IL-6 showed a significant increase 3 h after CFA stimulation.GlyCAM-1 was increased at 3 h, reached peak levels at 12 h, and gradually decreased thereafter. Levels of L-selectin bound to the plasma GlyCAM-1 changed over a similar time course, reached peak at 12 h after, and then began to decrease. The binding of L-selectin to plasma GlyCAM-1 was completely eliminated with the presence of ethyleneglycol-bis (b-aminoethylether) -N,N'-tetraacetic acid, showing the calcium dependency of this binding. These findings show that GlyCAM-1 release is enhanced by inflammatory stimulation and also suggest that released plasma GlyCAM-1 may trap, at least in part, solible L-selectin shed from stimulated leukocytes to neutralize each other.
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Report
(3 results)
Research Products
(3 results)
