| Co-Investigator(Kenkyū-buntansha) |
KAMEOKA Nubuhisa Kyushu Univ.Fac.Med research fellow, 医学部, 医員
SHIMIZU Shuji Kyushu Univ.Fac.Med assistant Prof., 医学部, 助手 (70274454)
KUROKI Syoji Kyushu Univ.Fac.Med assistant Prof., 医学部, 助手 (30215090)
牧野 一郎 九州大学, 医学部, 医員
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Research Abstract |
delta-Bilirubin, a nonenzymatic covalently bound complex with albumin, is nonenzymatically formed in serum and has a long half-life irrespective of hepatorenal function. The aim was to examine if delta-bilirubin reflects the duration of obstructive jaundice and efficacy of biliary drainage. Obstructive jaundice was developed in 17 dogs either for 4 (short term ; n=9) or 11 (long term ; n=8) days and then external biliary drainage was performed. Serum total, direct, and delta-bilirubin fractions were analyzed by high-performance liquid chromatography. delta-Bilirubin was not detectable in serum before biliary obstruction. The serum total and derect bilirubin levels increased rapidly and reached a plateau within 2 days after the biliary obstruction, whereas the concentration and proportion of delta-bilirubin gradually increased and significantly correlated with the duration of obstructive jaundice. Before biliary drainage, the proportion of delta-bilirubin was 28.9(]SY.+-。[)2.4% in the shortterm and 42.5(]SY.+-。[)2.4% in the long-term obstruction group. The decline indices of serum total, direct, and total minus delta-bilirubin in the short-term group (-0.17, -0.19, and -0.26, respectively) were significantly (P<0.05) greater than those in the long-term obstruction group (-0.10, -0.10, and -0.13). The decline index of delta-bilirubin itself was similar between the groups. In conclusion, the concentration and proportion of delta-bilirubin in serum reflect the duration of jaundice. Because delta-bilirubin is not excreted into bile and urine and is not toxic to organs, a decline index of total bilirubin minus delta-bilirubin, the excretable bilirubin fraction might be better to assess the efficacy of biliary drainge. Similar results were obtained from 21 human samples.
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