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RESEARCH FOR THE TRANSDIFFERENTIATION OF PANCREATIC CELLS TO HEPATOCYTES

Research Project

Project/Area Number 07671412
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionMIYAZAKI MEDICAL COLLEGE

Principal Investigator

IDE Hideyuki  MIYAZAKI MEDICAL COLLEGE,SURGERY I,ASSISTANT PROFESSOR, 医学部, 講師 (80264381)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsPANCREATIC HEPATOCYTE / CHOLESTEROL 7alpha-HYDROXYLASE / BILE ACID / COPPER DEFICIENCY / 銅欠乏 / pancreatic hepatocyte / コレステロール代謝
Research Abstract

Background & Aims : Hepatocyte-like cells have been observed in the pancreas of the rat. We examined the bile acid biosynthetic function of these cells to determine whether they are real hepatocytes. This study investigated the gene expression of two liver-specific enzymes regulating bile acid biosynthesis (cholesterol 7alpha-hydroxylase and DELTA^4-3-ketosteroid 5beta-reductase) in the hepatocyte-like cells.
Methods : Hepatocyte-like cells were induced in the rat pancreas by a copper depletion-repletion regimen. The expression of messenger RNA for cholesterol 7alpha-hydroxylase and DELTA^4-3-ketosteroid 5beta-reductase was detected using the reverse transcriptase polymerase chain reaction. The initial amount of cholesterol 7alpha-hydroxylase messenger RNA was quantified using the comeptitive polymerase chain reaction method.
Results : Messenger RNA was expressed in the pancreatic hepatocyte-like cells for cholesterol 7alpha-hydroxylase and DELTA^4-3-ketosteroid 5beta-reductase. A circadian rhythm of cholesterol 7alpha-hydroxylase messenger RNA expression was identified.
Conclusions : This study confirms that the hepatocyte-like cells produce bile acids.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Ando, Y., Ide, H., et al: "Expression of cholesterol 7α-hydroxylase and Δ^4-3-ketosteroid 5β-reductase genes in hepatocvte-like rat pancreatic cells." (submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideyuki Ide, Vadrevu Subbarao, Janardan K.Reddy, and M.Sambasiba Rao: "Formation of Ductular Structures in vitro by Rat Pancreatic Epithelial Oval Cells." Experimental Cell Research. 209. 38-44 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Ide, A.V.Yeldandi, J.K.Reddy, M.S.Rao: "Increased expression of sulfated glycoprotein 2 and DNA fragmentation in the pancreas of copper-deficient rats." Toxicology and Applied Pharmacology. 126. 174-177 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary

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Published: 1995-04-01   Modified: 2016-04-21  

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