| Project/Area Number |
07671487
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | KITASATO UNIVERSITY |
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Principal Investigator |
MASUDA Takashi Kitasato Univ.School of Medicine, Assistant Profess, 医学部, 講師 (30165716)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Kiyotaka Kitasato Univ.School of Medicine, Assistant Professok, 医学部, 講師 (40225933)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | intracellular free Mg^<2+> / ^<31>P NMR / Mg transport / intracellular free magnesium / ^<31>P NMR specfroscopy / Mg^<2+> / Pi exchanger / linear prediction Z-fransform / 心筋 / マグネシウム / ミトコンドリア / エネルギー代謝 / カルシウム / 心仕事効率 / 心機能 / 酸化還元状態 |
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| Research Abstract |
The aim of this study was to measure the concentration of intracellular free magnesium (free [Mg<@D12+@>D1] i) in isolated rat heart by analyzing<@D131@>D1P NMR spectra obtained from the linear prediction z-transform method (LPZAR), and to investigate the effect of extracellular inorganic phosphate ([Pi] o) on myocardial Mg metabolism. Free [Mg<@D12+@>D1] i of Langendorff perfused hearts was assessed by <@D131@>D1P NMR spectroscopy using LPZAR.The ionic enviroment was altered by changing [Pi] o from 1.2 to 5.0 mM in Krebs-Henseleit buffer during heart perfusion (n=6). Chenges in free [Mg<@D12+@>D1] i were calculated from the frequency difference between alpha-and beta-ATP in the<@D131@>D1P NMR spectrum. The spectra obtained using LPZAR provided peaks sharp enough for the calculation of free [Mg S12+ E1] i and distinct chemical shift of beta-peak of ATP enabled to estimate the change of free [Mg<@D12+@>D1] i. Wten [Pi] o was increased from 1.2 mM of normal concentration to 5.0 mM and then restored to 1.2 mM,free [Mg S12+ E1] i decreased significantly from 0.525(]SY.+-。[)0.048 mM to 0.407(]SY.+-。[)0.025 mM and returned to the control level of 0.601(]SY.+-。[)0.053 mM.The net movement of free Mg<@D12+@>D1 may involve Mg<@D12+@>D1-Pi exchange mechanism across the plasma membrane in rat heart, as the increase in extracellular [Pi] resulted in the decrease in free [Mg<@D12+@>D1] i.
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