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Study of factors that regulates site-specific brain metastasis

Research Project

Project/Area Number 07671500
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionUNIVERSITY OF TOKYO

Principal Investigator

FUJIMAKI Takamitsu  The University of Tokyo, Department of Neurosurgery, Faculty of Medicine, Associate, 医学部・附属病院, 助手 (80251255)

Co-Investigator(Kenkyū-buntansha) IRIMURA Tatsuro  The University of Tokyo, Department of Chemical Toxicity and Immunology, Faculty, 薬学部, 教授 (80092146)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsmelanoma / brain metastasis / site-specific brain metastasis / TGF-beta
Research Abstract

Mouse B16 melanoma cells which originally does not grow in the brain parenchyma were selected by modified Boyden chamber method for adhesiveness to mouse brain endotherial cells. Intracarotid injection of these cell produced intraparenchymal growth in some mice. However the difference in the pattern of growth of these cells from that of parent cell line was not significant, i. e. the adhesiveness to brain endotherial cells is not a major factor to determine site specific metastasis to brain parenchyma.
Six human melanoma cell lines derived from cutaneous lymph node or brain metastases and melanoma cells isolated from fresh surgical specimens of two primary cutaneous melanomas, two lymph node metastases and two brain metastases (each from a different patient) were injected into the subarachnoid space of nude mice. All melanomas produced growths in the leptomeninges, but only melanoma cells isolated from brain metastases infiltrated into and grew in the brain parenchyma of nude mice. The growth in vitro of human melanoma cells in the presence of TGF-beta inversely correlated with potential for brain parenchyma metastasis. These data suggest that infiltrative growth of melanoma cells into the brain parenchyma after intracistemal injection is a good model to assess the capacity of each melanoma to metastasize to brain parenchyma. These data also suggest that melanoma brain parenchymal metastases are produced by unique cells that may be resistant to the antiproliferative effects of TGF-beta.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Fujimaki T,Kirino T.Fidler IJ.,他: "Selective growth of human melanoma cells in the brain parenchyma of nude mice." Melanoma Res. 6巻. 363-371 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujimaki T,Kirino T: "Selective growth of human melanoma cells in the brain parenchyma of nude mice." Melanoma Res.6. 363-371 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujimaki T,Kirino T,Fidler IJ他: "Selective growth of human melanoma cells in the brain parenchyma of nude mice." Melanoma Res. 6巻. 363-371 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 藤巻高光: "続癌転移研究マニュアル マウス頚動脈腫瘍注入モデル" 金芳堂(印刷中), (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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