| Project/Area Number |
07671512
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | SHIGA UNIVERSITY OF MEDICAL SCIENCE |
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Principal Investigator |
NAKASU Yoko Shiga University of Medical Science, Department of Neurosurgery, Assistant Professor, 医学部, 講師 (10135478)
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| Co-Investigator(Kenkyū-buntansha) |
MORIKAWA Shigehiro Shiga University of Medical Science, Department of Neurosurgery, Associate Profe, 助教授 (60220042)
NAKASU Satoshi Shiga University of Medical Science, Department of Neurosurgery, Assistant Profe, 医学部, 講師 (00135477)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | bicuculline / blood-brain barrier / diffusion weighted image / epilepsy / lactate / magnetic resonance / spectroscopy / 脳浮腫 / ラット / Chemical shift imaging / Diffusion-weighted imaging / Epilepsy / Magnetic resonance imaging / Magnetic resonance spectroscopy / Rat / Status epilepticus |
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| Research Abstract |
Purpose : To observe metabolic maps of lactate and NAA by proton nuclear magnetic resonance (NMR) in vivo in the rat brain during the acute stage of hemispheric seizures.
Methods : Adult male sprague-Dawley rats were anaesthetized with isoflurane, and were examined using a 2.0-T CSI Omega system. Seizures were elicited through transient opening of the blood-brain barrier (BBB) in the left hemisphere by injection of hyperosmolar mannitol solution into the left internal carotid artery, following an intravenous injection of bicuculline methiodide. Both hemispheres were then observed from an axial view before and after the induction of seizures using the three-dimensional chemical shift imaging (CSI) techinique. Proton CSI data were obtained from a 40 * 40 mm2 FOV,divided into 8 * 8 matrix, using water suppression, fat suppression, and outer volume suppression, thus creating a metabolic map. Opening of the BBB was confirmed as a transient increase in signal intensity in the left hemisphere
… More
on T2-weighted MR images.
Results : Neither intravenous injection of bicuculline methiodide nor transient opening of the BBB per se caused seizure activity. The rats developed tonic-clonic seizures with the transient opening of the BBB following an injection of bicuculline methiodide. The left hemisphere showed periodic spikes on a scalp EEG about 15 minutes after the opening. Metabolic images showed an accumulation of lactate in the left cerebral hemisphere one and three hours after seizure induction. Metabolic images of lactate, however, detected no abnormality in the hemisphere 24 hours after seizure. No clear concurrent changes in the NAA map were obsereved in this study. Cerebellar hemispheres presented no changes on lactate or NAA metabolic images
Conclusions : We found a transient accumulation of lactate in the cerebral hemisphere due to abnormal neuronal excitation after elicitation with bicuculline methiodide. The metabolic maps were obtained in vivo and in a non-invasive manner. We suggest that lactate can be a reversible indicator in NMR metabolic mapping, showing the focus of abnormal neuronal excitation in the acute stage of clinical seizures. Less
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