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Cloning of immortalizing and transforming genes of type 1 astroglia

Research Project

Project/Area Number 07671515
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionOsaka University

Principal Investigator

HIRAGA Shoju  Osaka University Medical School, Assistant Professor, 医学部, 助手 (40243232)

Co-Investigator(Kenkyū-buntansha) HAYAKAWA Toru  Osaka University Medical School, Professor, 医学部, 教授 (20135700)
OHNISHI Takanori  Osaka University Medical School, Assistant Professor, 医学部, 助手 (70233210)
瀧 琢有  大阪大学, 医学部・附属病院, 医員
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsastroglia / immortalization / gene / transformation / carcinogenesis / Astrocyte / Immortalization / Gene / Cloning / Carcinogenesis
Research Abstract

We have obtained 5 immortalized astroglial cells of which growth and morphological characters are indistinguishable from normla ones but express carcinoembryonic tenascin and have strong motility to glial motility factor. The cells have intermediate characters between transformed and normal cells. We investigated genes responsible for immortalization and transformation of type 1 astroglia. Differental hybridization was performed between the normal and immortalized cells and we obtained 26 genes. We excluded fibronectin, intergrins, heat shock proteins and mortalin genes by gene homology survey and 6 genes which have unreported DNA sequences were candidated for astroglial immortalization. Likewise, 4 genes were subcloned and candidated for the transformation of astroglia using differential display method. The former genes are introduced to senescent cells and recovery from the senescence of the cells were now under investigation. The latter subclones are also introduced to immortalized cells. We reported that tumor suppressor p53 gene correlated with transformation and not with immortalization of astroglia. Moreover, we are subcloning human homologic genes to the above mentioned genes. Thus, investigation of our astroglial carcinogenesis model contribute to simplifying and elucidating pathophysiology of human glioma development and progression.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] S. Hiraga, et al: "Establishment of spontaneously immortalized rat type 1 astroglial cell lines : the role of p53 in astroglial carcinogenesis." Glia.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Hiraga, et al: "Transformation of type 1 astrocytes with N-ethyl-N-nitrosourea : Establishment of an in vitro system and the role of the p53 gene" Glia.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Ohnishi, et al: "Fibronectin-mediated cell migration promotes glioma cell invasion through chemokinetic activity." Clin. Exp. Metastasis. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Taki, et al: "Antisense inhibition of RAD51 enhances radiosensitivity." Biochem. Biophys. Res. Commun.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Izumoto, et al: "Gene expression of neural cell adhesion L1 in malignant gliomas and biological significance of L1 in glioma invasion." Cancer Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Izumoto, et al: "Microsatellite instability and mutated type II transforming growth factor-b receptor gene in gliomas." Cancer Letters.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Ohnishi, et al: "Brain Tumor Research and Therapy" Springer-Verlag, Tokyo,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Izumoto, et al: "Brain Tumor Research and Therapy" Springer-Verlag, Tokyo,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiraga S,Arita N,Ohnishi T,Izumoto S,Taki T,Higuchi M,Iwaisako K,Sakoda S,Yamamoto Y,Hayakawa T: "Establishment of spontaneously immortalized rat type 1 astroglial cell lines : the role of p53 in astroglial carcinogenesis" Glia. 18. 185-199 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiraga S,Arita N,Ohnishi T,Izumoto S,Taki T,Yamamoto H,Higuchi M,Hayakawa T: "Transformation of type 1 astrocytes with N-ethyl-N-nitrosourea : -Establishment of an in vitro system and the role of the p53 gene" Glia. 13. 51-63 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohnishi T,Arita N,Hiraga S,Izumoto S,Hayakawa T: "Fibronectin-mediated cell migration promotes glioma cell invasion through chemokinetic activity" Clin Exp Metastasis. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taki T,Ohnishi T,Yamamoto A,Hiraga S,Arita N,Izumoto S,Hayakawa T,Morita T: "Antisense inhibition of the RAD51 enhancs radiosensitivity" Biochem Biophys Res Comm. 223. 434-438 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Izumoto S,Ohnishi T,Arita N,Hiraga S,Taki T,Hayakawa T: "Gene expression of neural cell adhesion molecule L1 in malignant gliomas and biological significance of L1 in glioma invasion" Cancer Res.56. 1440-1444 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Izumoto S,Arita N,Ohnishi T,Hiraga S,Taki T,Tomita N,Ohue M,Hayakawa T: "Mictosatellite instability and mutated type II transforming growth factor-b receptor gene in gliomas" Cancer Letters. 112. 251-256 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Izumoto S,Arita N,Yamasaki M,Hiraga S,Ohnishi T,Hayakawa T: "A new mutation of LlCAM gene in an X-linked hydrocephalus family" Child's Nerv.System. 12. 742-747 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Izumoto S,Arita N,Ohnishi T,Hiraga S,Taki T,Hayakawa T: "Homozygous deletions of p161NK4A/MTS1 and p151NK4B/MTS2 genes in glioma cells and primary glioma tissues." Cancer Letters. 97. 241-247 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamasaki M,Arita N,Hiraga S,Izumoto S,Morimoto K,Nakatani S,Fujitani K,Sato N,T Hayakawa: "X-linked hydrocephalus in Japan -clinical and neuroradiological study-" J Neurosurg. 83. 50-55 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Higuchi M,Ohnishi T,Arita N,Hiraga S,Hayakawa T: "Leptomeningeal dissemination of malignant glioma : immunohistochemical analysis of tenascin and glial fibrillary acidic protein" Clin Neuropathol. 14. 223-227 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Izumoto S,Arita N,Ohnishi T,Hiraga S,Taki T,Hayakawa T: Brain Tumor Research and Therapy (ed by Nagai M) : "Expression of L1 CAM in brain tumors : Immunohistochemical Study using Anti-L1 CAM antibodies". Spinger-Verlag, Tokyo, 103-107 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohnishi T,Izumoto S,Arita N,Hiraga S,Taki T,Hayakawa T: Brain Tumor Research and Therapy (ed by Nagai M) : "Expression and biological function of L1 cell adhesion molecule in malignant glioma cells". Spinger-Verlag, Tokyo, 103-107 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Hiraga,et al: "Establishment of spontaneouesly immortalized rat type 1 astroglial cell lines : the role of p53 in astroglial carcinogenesis." Glia. 18. 185-199 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Hiraga,et al: "Transformation of type 1 astrocytes with N-ethyl-N-nitrosourea : Establishment of an in vitro system and the role of the p53 gene" Glia. 13. 51-63 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Ohnishi,et al: "Fibronectin-mediated cell migration promotes glioma cell invasion through chemokinetic activity." Clin.Exp.metastasis. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Taki,et al: "Antisense inhibition of RAD51 enhances radiosensitivity." Biochem.Biophys.Res.Commun.223. 434-438 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Izumoto,et al: "Gene expression of neural cell adhesion L1 in malignant gliomas and biological significance of L1 in glioma invasion." Cancer Res.56. 1440-1444 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Izumoto,et al: "Microsatellite instability and mutated type II transforming growth factor-b receptor gene in gliomas." Cancer Letters. 112. 251-256 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Ohnishi,et al: "Brain Tumor Research and Therapy" Springer-Verlag,Tokyo, 10 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Izumoto,et al: "Brain Tumor Research and Therapy" Springer-Verlag,Tokyo, 5 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiraga S,et al.: "Transformation of type 1 astrocytes with N-enthyl-N-nitrotosoured" GLIA. 13. 51-63 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamasaki M,et al.: "X-linked hydrocephalus in Japan Clinical and neuroradiological study." J.Neurosurgery. 83. 50-55 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Higuchi M,et al.: "Leptomeningeal dissemination of malignant glioma immunohistochemical analysis of tenascin and glial fibrillary acidic protein" Clin.Neuropathology. 14. 223-227 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Izumoto S,et al.: "Homozygous deletion of p16INK4A/MTS1 and p15INK4B/MTS2 genes in glioma cells and primary glioma tissues." Cancer Letters. 97. 241-247 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Izumoto S,et al.: "Gene expression of neural cell adhesion molecule L1 in malignant gliomas and biological significance of L1 in glioma invasion" Cancer Reserach. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Izumoto S,et al.: "A new mutation of L1CAM gene in a X-linked hydrocephalus family" Child's Nervous System. (in press).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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