• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of a differentiation-related gene in the rat central nervous system using differential display method

Research Project

Project/Area Number 07671528
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionSAGA MEDICAL SCHOOL

Principal Investigator

MINETA Toshihiro  SAGA MEDICAL SCHOOL, 医学部, 助手 (20264187)

Co-Investigator(Kenkyū-buntansha) FUKUYAMA Kouzou  佐賀医科大学, 医学部, 助手 (60238516)
TABUCHI Kazuo  佐賀医科大学, 医学部, 教授 (50116480)
桃崎 宣明  佐賀医科大学, 医学部, 講師 (30239587)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsneuron / oigodendrocyte / astrocyte / glial differentiation / neuronal differentiation / VASP / differential display / neuronal differentiotion / glial differentiotion / VASP
Research Abstract

During development of the central nervous system (CNS), a large number of genes are expressed and interact with each other. Although the functions of certain genes involved in CNS development have been described, the process of development and differentiation in the CNS is not clearly understood. We have used the differential display method to identify genes which are differentially expressed during CNS development. Using this approach, we have identified a novel rat gene that codes for a protein of 42 kDa (RNB6) and is expressed in the developing embryonic rat brain. The level of expression of RNB6 peaks on postnatal day 1 in brain tissue and gradually decrease thereafter. The predicted amino acid sequence of RNB6 shares a 45% homology with the human actin-associated protein, vasodilator-stimulated phosphoprotein (VASP) and RNB6 contains a GlyProProProProPro (GP_5) motif, that is highly conserved feature of VASP family members. VASP functions through its GP_5 motifs as a ligand for profilin, a signal transducer for actin filament assembly and therefore is thought to control cellular motility through profilin-mediated microfilament assembly. The shared homology and common GP_5 motif of RNB6 and VASP suggest that RNB6 may take part in cellular motility during development of the CNS.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Mineta,T.: "Attenuated multi-mutated herpes simplex virus-1 for the treatment of malignant gliomas." Nuture Medicine. 1. 938-943 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yazaki,T.: "Inhibition of angiogenesis and growth of human non-malignant and malignant meningiomas by TNP-470." J Neuro-oncol.23. 23-29 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 峯田寿裕: "組み換え型単純ヘルペスウイルス1型による悪性脳腫瘍の実験的治療." 脳神経外科. 23. 285-292 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mineta,T.: "Braim Tumor Research and Therapy" Springer-Verlag Tokyo, 6 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mineta T.et al.: "Attenuated multi-mutated herpes simplex virus-1 for the treatment of malignant glioma" Nature medicine. vol.1. 938-943 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yazaki T.et al.: "Inhibition of angiogenesis and growth of human non-malignant and malignant meningioma by TNP-470" J.Neuro-oncology. vol.23. 23-29 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohta S.et al.: "Identification of a differentiation-related gene in the rat central nervous system using differential display method." Neuroimmunological Research. vol.9. 64-67 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mineta.T: Experimental therapy for malignant brain tumors using herpes simplex virus type 1. Brain tumor research and therapy. Nagai M.Edit.Springer-velag Tokyo, 409-414 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mineta,T.: "Attenuated multi-mutated herpes simplex virus-1 for the treatment of malignart glicma," Nature medicine. 1. 938-943 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yazaki,T.: "Inhibtion of angiogenesis and growth of human nonnalignant and nalignant meningl oma by TNP-410" J.Neuro-oncology. 23. 23-29 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 太田昭一郎: "Diffevential display法による中枢神経系分化関連遺伝子の同定" 神経免疫研究. 9. 64-67 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mineta,T.: "Brain Tumar Research and Therapy Nagai,M,Ed" Springer-Verlag Tokyo, 6 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mineta,T.: "Attenuated multi-mutated herpes simplex virus-1 for the treatment of malignant gliomas." Nature Medicine. 1. 938-943 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yazaki,T.: "Inhibition of angiogenesis and growth of human non-malignant and malignant meningiomas by TNP-470." J Neuro-oncol.23. 23-29 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 峰田寿裕: "組み換え型単純ヘルペスウイルス1型による悪性脳腫瘍の実験的治療." 脳神経外科. 23. 285-292 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mineta,T.: "Braim Tumor Research and Therapy" Springer-Verlag Tokyo, 6 (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi