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Neuronal death and sphingolipid following occlusion of the middle cerebral artery

Research Project

Project/Area Number 07671543
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionTeikyo Univ.School of Med.

Principal Investigator

KUBOTA Masaru  Teikyo Univ.School of Med., Neurosurgery, Assistant Prof., 医学部, 講師 (30246061)

Co-Investigator(Kenkyū-buntansha) NARITA Koji  Teikyo Univ School of Med., Neurosurgery, Assistant, 医学部, 助手 (90237602)
KANEMITSU Hideaki  Teikyo Univ School of Med., Neurosurgery, Assistant Prof, 医学部, 講師 (10129992)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
KeywordsFocal cerebral ischemia / sphingomyelin / middle cerebral artery / programd cell death / sphingolipid / forebrain ischemia / 一過性前脳虚血 / 神経細胞死 / 薄層クロマトグラフィー / 局所脳虚血 / セレブロシド / セラミド / ガングリオシド / スフィンゴミエリン / 脂肪酸
Research Abstract

Focal cerebral ischemia was induced in anesthetized rats by occluding the stem of the proximal middle cerebral artery. To better define the sphingolipid metabolism during focal brain ischemia, levels of ceramide, sphingomyelin, cerebroside and gangliosides were determined in rat cerebral cortex during focal ischemia produced by occlusion of the middle cerebral artery. Sphingomyelin began to decrease at 2 hours of ischemia and continued to decrease for 96 hours. In contrast, ceramide increased at 6 hours and increased to 4.2-fold at 96 hours after ischemia, and the fatty acid composition of ceramide was solely nonhydroxylated fatty acid similar to sphingomyelin. Hydroxylated fatty acid-linked cerebroside decreased at 6 hours of ischemia, whereas any significant decrease of non-hydroxylated fatty acid-linked cerebroside didn't occur for 96 hours of ischemia. There were no measurable changes in the levels of gangliosides. These results suggested that ceramide was produced in the cerebral cortex by the breakdown of sphingomyelin during early ischemia.
Recently ceramide generated by sphingomyelin degradation has been described as a second messenger involved in programd cell death, cell growth and differentiation in HL-60 leukemic cells. We studied on the differences in sphingomylin levels in gerbil hippocampus induced by lethal or sublethal ischemia using the transient forebrain ischemia-reperfusion gerbil model. Sphingomyelin was separated by thin layr chromatography (TLC) from hippocampal total lipids and analyzed by gas liquid chromatography (GLC). The sphingomyelin levels during recirculation after lethal ischemia (a 5-min.of ischemia) were significantly decreased at 30 min.and then returned to the control level at 60 min. The recirculation after a sublethal ischemia (2 min ischemia and 5 min.ischemia after acquired ischemic tolerance) did not induce any changes in sphingomyelin levels.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 久保田 勝: "局所脳虚血とスフィンゴリピド-増加するセラミドの由来について" 脳神経. 47. 756-761 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kubota M: "Shingomyelin changes in rat cerebral cortex during focal ischemia" Neurcl.Res. 18. 337-341 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 久保田 勝: "遅発性細胞死における海馬FFAとスフィンゴミエリンの変動" 脂質生化学研究. 38. 115-118 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kubota M: "Arachidonic acid accumlation following transient forebran ischemia in the gerbil nippocampus with tolerance." J. Cerebral Blood Flow and Metabalism. 17(suppl). S427 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 中根 一: "砂ネズミ一過性前脳虚血後の海馬スフィングミエリンの変動" Brain Hypoxia. 11. 21-26 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kubota M: "Mild hypothemia reduces the rate of metabolism of arachidonic acid following postischemic reperfusion" Brain Res. 779. 297-300 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kubota M,Narita K,Nakagomi T,Tamura A,Shimasaki H,Ueta N,Yoshida S: "Sphingomyelin changes in rat cerebral cortex during focal ischemia." Neurol Res. 18. 337-341 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kubota M,Nakane M,Narita K,Nakagomi T,Tamura A,Hisaki H,Shimasaki H,Ueta N: "Arachidonic acid accumulation following transient forebrain ischemia in gerbil hippocampus with induced tolerance." J Cerbral Blood Flow and Metabol. 17 (suppl.1). S427 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakagomi T,Kanemitsu H,Takagi K,Kubota M,Narita K,Nagashima H,Tamura A: "Increased protein synthesis in the ipsilateral substantia nigra after focal ischemia in the rat." J Cerbral Blood Flow and Metabol. 17 (suppl.1). S51 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakane M,Kubota M,Nakagomi T,Tamura A,Hisaki H,Shimasaki H,Ueta N: "Sphingomyelin levels of hippocampus after transient forebrain ischemia in the gerbil." J Cerbral Blood Flow and Metabol. 17 (suppl.1). S426 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kubota M,Nakane M,Narita K,Nakagomi T,Tamura A,Hisaki H,Shimasaki H,Ueta N: "Mild hypothermia reduces the rate of metabolism of arachidonic acid following postischemic reperfusion." Brain Res. 779. 297-300 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kubota M,Nakane M,Narita K,Ishii T,Nakayama T,Nakagomi T,Tamura A,Hisaki H,Shimasaki H,Ueta N: Sphingomyelin changes during transient forebrain ischemia. Differences between lethal and sub-lethal ischemia. 11th International Congress of Neurological Surgery.Monduzzi Editore, Bologna (Italy), 1845-1849 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kubota M: "Archidonic acid accumlation following transient forebrain ischemia in gerbil hippocampus with induced tolerance" J.CBF and Matabolism. 17(Suppl). S427 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakane M: "Sphingomyelin levels of hippocampus after transient forebrain ischemia in the gerbil" J.Cerebral Blood Flow and Metabolism. 17(suppl). S426 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 中根 一: "砂ネズミ一過性前脳虚血後のスフィンゴミエリンの変動" Brain Hypoxia 1997. 11. 21-26 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 中根 一: "セラミドと一過性前脳虚血後の海馬遅発生神経細胞死" 脳循環代謝. 9. 72-73 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kubota M: "Mild hypothermia reduces the rat of metabolism of arachidnic acid following postischemic reperfusion" Brain Res. 779. 297-300 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kubota M, et al.: "Ceramid accumulates in rat cerebral cortex during ischemia" J CBF and Metabol. 15(Supple). S334 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久保田 勝,他: "局所脳虚血とスフィンゴリピト;増加するセラミドの由来について" 脳と神経. 47. 756-761 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 成田考而,久保田勝他: "ラットMCA閉塞モデル大脳皮質における遊離脂肪酸の経時的変動" Brain Hypoxia. 9. 43-51 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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