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THE PATHOGENESIS OF MYELOSCHISIS

Research Project

Project/Area Number 07671546
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionJIKEI UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

KIKUCHI Tetsuro  JIKEI UNIV.SCHOOL OF MED., DEPARTMENT OF NEUROSURGERY,LECTURER, 医学部・脳神経外科, 講師 (60177798)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Koichi  JIKEI UNIV.SCHOOL OF MED., DEPARTMENT OF NEUROSURGERY,ASSISTANT, 医学部・脳神経外科, 助手 (90246413)
TANAKA Hideaki  JIKEI UNIV.SCHOOL OF MED., DEPARTMENT OF NEUROSURGERY,ASSISTANT, 医学部・脳神経外科, 助手 (70227164)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
KeywordsSPINA BIFIDA / GFAP / GLIOSIS / GROWTH FACTOR / AMNIOTIC FLUID / APOPTOSIS / マウス / NFP / グリア / ニューロン
Research Abstract

Immunohistochemical Analysis of GFAP and NFP in the Fetal Spinal Cord
We analyzed the expression of GFAP and NFP in the fetal spinal cord of the normal and Splotch-Delayd (Spd) mice suffering from myeloschisis. A lot of GFAP positive cells were seen in the spinal cord of the Spd mice especially on the dorsal side, while in that of normal mice, few positive cells were detected, suggesting the existence of the gliosis in the myeloschisis. NFP positive cells were not detected.
The Expression of Growth Factors by RT-PCR
We analyzed the mRNA expression of growth factors (TGF,FGF,PDGF) in the fetal spinal cord of the normal and Spd mice by RT-PCR.There was no remarkable difference in the expression of these growth factors between normal spinal cord and myeloschisis.
Immunohistochemical Analysis of p53
We analyzed the expression of p53 protein in myeloschisis and normal spinal cord using immunohistochemical staining. In myeloschisis, p53 positive cells were shown while in normal spinal cord, positive cells do not exist. Although this result was not reproducible. We are continuing to investigate the difference in p53 expression between normal spinal cord and myeloschisis.
Apoptotic Cells
We are now investigating the existence of apoptotic cells in myeloschisis by Tunel method.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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