| Project/Area Number |
07671596
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KASHIWAGUCHI Shinji The University of Tokushima School of Medicine University Hospital, lecturer, 医学部・附属病院, 講師 (30224398)
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| Co-Investigator(Kenkyū-buntansha) |
UMEHARA Takashi The University of Tokushima School of Medisine, assistant, 医学部, 助手 (20263816)
TAKEDA Yoshitsugu The University of Tokushima School of Medisine University Hospital, assistant, 医学部・附属病院, 助手 (20243694)
加藤 真介 徳島大学, 医学部, 講師 (30243687)
井形 高明 徳島大学, 医学部, 教授 (80108860)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | articular cartilags in growth stagengs / repair of articular cartilage / apoptosis / electrophoresis of DNA / 成長軟骨板 / 移植 / 関節軟骨 |
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| Research Abstract |
Transplants of the apophyseal growth plate as autografts obtained from the ilium of Lewis rats at 4 weeks of age were inserted into full-thickness defects in the patellofemoral articular surface of same rats. As controls, similar surface defects were induced but no transplants were inserted. Femoral specimens including transplants (n=18) and defects (n=6) were cut and fixed in formarin and paraffin embedded. The tissue sections were studied by in situ end-labeling procedure at varing intervals up to 4 weeks in order to detect the apoptosis.
No staining of cells with morphological characteristics of apoptosis was demonstrated in tissues fo growth plate until the incorporation of the grafts to the defects. But apoptotic cells were detected in bone marrow under the graft at 1 and 2 weeks after transplantation. In the control specimens, apoptosis was not detected in bone marrow of the defect.
These findings suggested that some apoptotic inducers in the grafted growth plate simulated apoptosis of the cells in bone marrow in the articular cartilage defect.
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