| Project/Area Number |
07671598
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
MATSUMOTO Tomoko Nagasaki University, School of Medicine, Hospital, Assistant, 医学部附属病院, 助手 (30239107)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Shunichi Nagasaki University, School of Medicine, Professor, 医学部, 教授 (30200679)
岩崎 勝郎 長崎大学, 医学部, 教授 (60039542)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | chondrocytes / TGF-b / signal transduction / TGF β receptor / chondrocyte / two-hybrid cloning |
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| Research Abstract |
Transforming growth factor (TGF) -b promotes the proliferation and differentiation of chondrocytes, and it is assumed to play a role in the repair of cartilage tissue. The mechanism of signal transduction of TGF-b is still unknown, although type I,II and III receptors of TGF-b have been cloned. TGF-b stimulates the synthesis of DNA in chondrocytes unlike the other cells, suggesting signal transduction of TGF-b in chondrocytes is different from the others. We found that TGF-b upregulated the induction of c-fos gene in chondrocytes, and that TGF-b response element of upstream of c-fos gene is -403 to -222 using CAT assay. We have made c-DNA library of rat articular chondrocytes and tried to clone the protein intermediating the signal transduction of TGF-b by two hybrid system. Several colonies were obtained and they are screening now, however, no positive data are obtained. Further studies are needed to find a novel protein.
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