| Project/Area Number |
07671602
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Oita Medical University |
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Principal Investigator |
TORISU Takehiko Oita Medical University, Department of Orthopaedic Surgery, Associate Professor, 医学部, 助教授 (70145382)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Seiji Oita Medical University, Department of Human Biology Professor, 医学部, 教授 (60034956)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Synovial membrane / Lymphatics / Enzyme-histochemistry |
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| Research Abstract |
The response to intra-articularly injected plastic particles and their drainage on reactive granulation in the rat knee joints was examined by light and electron microscopy. These knee joints and associated iliac lymph nodes were excised after 5 minutes and also after intermediate periods until 3 months after injection of latex (1mum) or fluoresbrite particles (0.2mum or 10mum). Particles in the lymphatic or blood vessels were successfully substantiated by an enzymc-histochemical method (5'-nuclcotidase staining). Five minutes after the injection, most of the particles were scattered on the surface of the synovial membrane, and some particles were phagocytosed by synovial lining cells. After 5 hours, neutrophils phagocytosed particles which adhered to fibrin in the joint cavity. Twelve hours later, after the neutrophils had died, those same particles were phagocytosed by macrophages in the joint cavity. After 1 day, fluoresbrite particles (0.2mum) phagocytosed by macrophages were found in the iliac lymph nodes. Whereas latex particles (1mum) were detected in the iliac lymph nodes after 3 days. Some fluoresbrite particles (10mum) were seen in the 5'-nucleotidase-positive lymphatic vessels in the synovial membrane. After 3 months, many macrophages full of particles formed granulation in the synovial membrane, and macrophages with phagocytosed particles were also seen increasingly in the iliac lymph nodes. Our findings suggested that ncutrophils and macrophages phagocytosed injected particles in the joint cavity, and that the macrophages brought the particles into the deep layr of the synovial membrane. Phagocytic macrophages also carried the particles to the iliac lymph nodes through lymphatic vessels in the synovial membrane. There were no morphological differences in the process of granulation and drainage between two sizes of plastic particles (1mum and 0.2mum) except the behavior of macrophages phagocytosing the particles.
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