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Cell Biological Study for the Mechanism of Osteogenesis by Electromagnetic Fields.

Research Project

Project/Area Number 07671606
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKagoshima University

Principal Investigator

MATSUNAGA Shunji  Kagoshima University, University Hospital, Assistant Professor, 医学部・附属病院, 講師 (90229500)

Co-Investigator(Kenkyū-buntansha) YATA Toshihiko  Kagoshima University, Associate Professor, 医学部, 助教授 (60166527)
SAKOU Takashi  Kagoshima University, Faculty of Medicine, Professor, 医学部, 教授 (10041295)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsElectromagnetic / Osteogenesis / Ca ion / intra cellular signaling / Osteoblast / 副甲状腺ホルモン
Research Abstract

The following cxperimental results were obtained by this research.
1.Changes in Ca-ion incorporation into cells by stimulation of electromagnetic field in vitro
Intracellular Ca-ion concentration in osteoblasts that underwent electromagnetic field stimulation was higher the that in control osteoblasts by 15% on average. This acceleration of Ca-ion incorporation was most efficient in the electromagnetic field of 100 Hz in frequency, 15 (sec in pulse width and 2.0 gauss of the magnetic field intensity.
2.Evaluation of in vivo osteogenesis-enhancing effect of electromagnetic field stimulation at cellular level
Osteogenesis measured by an osteometer significantly increased under stimulation by the electromagnetic field of 100 Hz in frequency, 15 (sec in pulse width and 2.0 gauss of the magnetic field intensity. These results reflected those obtained in the in vitro experiment in the present study. In the examination of activity of alkaline phosphatase, a marker of osteoblast, and proliferation of osteoblasts using AgNOR staining, elevation of the enzyme and enhancement of proliferation were shown under the optimum stimulation conditions of the electromagnetic field.
As the mechanism of enhancement of osteogenesis by stimulation with the electromagnetic field, these results demonstrated that Ca-ion uptake by cells was changed by the electromagnetic field stimulation, changes in functions at the cellular level such as synthesis of alkaline phosphatase and cell-proliferation were induced via intracellular signal transaction in osteoblasts, and osteogenesis was enhanced. The fact that osteogenesis, and growth and Ca-ion incorporation by osteoblasts were changed by conditions of electromagnetic field stimulation strongly suggests the effect of electromagnetic field on cells.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 松永 俊二: "電気刺激による骨形成の基礎と臨床" 日本生体電気刺激研究会誌. 10. 11-15 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 松永 俊二: "電気刺激による骨形成機序に関する実験的検討" 日本整形外科学会雑誌. 70. 1252-1252 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shunhj Matsunaga: "Osteogenesis by pulsing electromagnetic Fields (PEMFs) : Optimum stimulation setting." in vivo. 10. 351-356 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kouji Yonemori: "The effect of electrical stimulation on osteogenesis." Bone. 19. 173-180 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tetsuro Shimono: "The effects of piezoelectric poly-L-lactic acid films in promoting ossification in vivo." in vivo. 10. 471-476 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kosei Ijiri: "The effect of pulsing electromagnetic field on bone ingrowth into porous coated implant" Anticancer Research. 16. 2853-2856 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shunji Matsunaga: "Basic Research and Clinical application of Electrical Stimulation for Osteogenesis." The Journal of the Japanese Bio-Electrical Research Society. 10. 11-15 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shunji Matsunaga: "Experimental Study on Mechanism of Osteogenesis Induced by Electrical Stimulation." The Journal of the Japanese Orthopaedic Association. 70. 1252-1252 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shunji Matsunaga: "Osteogenesis by pulsing electromagnetic Fields (PEMFs) : Optimum stimulation setting." in vivo. 10. 351-356 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kouji Yonemori: "The effect of electrical stimulation on osteogenesis." Bone. 19. 173-180 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tetsuro Shimono: "The effects of piezoelectric poly-L-Lactic acid films in promoting ossification in vivo." in vivo. 10. 471-476 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kosei Ijiri: "The effect of pulsing electromagnetic field on bone ingrowth into porous coated implant." Anticancer Research. 16. 2853-2856 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shunji Mastunaga: "Osteogenesis by pulsing electromagnetic Fields(PEMFs):Optimum stimulation setting." in vivo. 10. 351-356 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kouji Yonemori: "The effect of electrical stimulation on osteogenesis" Bone. 19. 173-180 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tetsuro Shimono: "The effects of piezoelectric poly-L-lactic acid films in promoting ossification in vivo" in vivo. 10. 471-476 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kosei Ijiri: "The effect of pulsing electromagnetic field on bone ingrowth into porous coated implant." Anticancer Research. 16. 2853-2856 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 松永俊二: "電気刺激による骨形成の基礎と臨床" 日本生体電気刺激研究会誌. 10巻. 11-15 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 米盛公治: "電気刺激による骨形成機序に関する実験的検討" 日本整形外科学会雑誌. 70巻・8号. 1252- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松永俊二: "変動電磁場刺激の細胞増殖促進効果について" 日本生体電気刺激研究会誌. 9. 71-73 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 松永俊二: "電磁場刺激の骨形成促進機序に関する実験的および臨床的検討" 日本整形外科学会雑誌. 69(8). S1788- (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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