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GENETIC BACKGROUND OF DISTRACTION TISSUE NEOGENESIS

Research Project

Project/Area Number 07671630
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKinki University School of Medicine

Principal Investigator

HAMANISHI Chiaki  Kinki University of Medicine Assicetant Professor, 医学部, 助教授 (00164921)

Co-Investigator(Kenkyū-buntansha) 葭井 健男  近畿大学, 医学部・附属病院, 助手 (80268408)
北本 克則  近畿大学, 医学部・附属病院, 助手 (50258034)
戸谷 由樹  近畿大学, 医学部, 助手 (60278695)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsleg lengthening / bone formation / bisphosphonate / cartilage / c-fos / protein kinase C / osteoarthritis / 脚延長 / 仮骨 / 骨形成 / MCSF
Research Abstract

The concept of distraction tissue neogenesis has been unique and its genetic background has been surveyed using lengthened callus made in rabbit tibiae with immunohistochemical methods for c-fos, type I and II collagen, osteocalcin and osteonectine. Distracted callus was finally adovocated as the excellent in vivo model for analyzing both endochondral and membranous bone formation, and abnormal bone metabolism such as osteoporosis and treatments. Several methods such as introduction of bisphosphonate was revealed to be effective for the treatment of osteoporosis. Then the effect of compression on the articular cartilage has been studied using experimental osteoarthritic model made in rats by severance of all ligamentous connection. The proto-oncogen c-fos and c-Fos protein were observed during early period after operation in all layrs of articular cartilage, then destruction of the cartilage ensued. Secondary messenger protein kinase c (PKC) was also appeared during early phase of osteoarthritis although intraarticular administration of PKC-activator PMA inhibited both intiation and progression of osteoarthritis. PMA also appeared to inhibit the expression of c-Fos in all layrs. It then appeared that PKC activator inhibited c-fos expression and made cartilage mature and mechanically tough to the compression stress. The free radicals such as NO and SO appeared to be synthesized by the damaged cartilage and auto-destruction mechanism was also revealed.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Satsuma H, Hamanishi C, Hashima M, Tanaka S,: "Alpha and epsilon isozymes of protein kinase C in the chondrocytes in normal and early osteoarthritic articular cartilage" Excerp.Med.256 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyazaki H, Hamanishi C, Yoshii T, Tanaka S,: "Effect of a single intravenous dose of bisphosphonate(pamidronate)on tibial cortical bone after lengthening" Acta Medica Kinki Univ.22(2):. 155-158 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyagi I, Kikuchi H, Hamanishi C, Tanaka S: "Auto-destruction of the articular cartilage and free radical mediators" J.Lab.Clin.Med. 131. 146-150 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Satsuma C.Hamanishi, M.Hashima S.Tanaka: "Immunohistochemical localization of cartilage-specific and non-specific proteoglycans in experimental osteoarthritic articular cartilage in rats" Advance in Osteoarthritis,Springer Verlag. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.Hamanishi H.Miyazaki, H.Satsuma S.Tanaka: "Protein kinase C-activator inhibits progression of osteoarthritis by iphibiting c-Fos protein expression in articular chondrocytes" Advance in Osteoarthritis,Springer Veriag. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Hashima, C.Hamanishi, S.Tanaka: "Muscarinic receptor-mediated Ca++control, protein kinase C activity and proteoglycan release and synthesis in articular chondrocytes" Advance in Osteoarthritis, Springer Verlag. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Satsuma H,Hamanishi C,Hashima M,Tanaka S: "Alpha and epsilon isozymes of protein kinase C in the chondrocytes in normal and early osteoarthritic articular cartilage" Excerp.Med.32. 256 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyazaki H,Hamanishi C,Yoshii T,Tanaka S: "Effect of a single intravenous dose of bisphosphonate (pamidronate) on tibial cortical bone after lengthening" Acta Medica Kinki Univ.22 (2). 155-158 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyagi I,Kikuchi H,Hamanishi C, Tanaka S: "Auto-destruction of the articular cartilage and free radical mediators" J.Lab.Clin.Med. 131. 146-150 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Satsuma C.Hamanishi, M.Hashima S.Tanaka: "Immunohistochemical localization of cartilage-specific and non-specific proteoglycans in experimental osteoarthritic articular cartilage in rats" Advance in Osteoarthritis, Springer Verlag. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.Hamanishi H.Miyazaki H.Satsuma S.Tanaka: "Protein kinase C-activator inhibits progression of osteoarthritis by inhibiting c-Fos protein expression in articular chondrocytes" Advance in Osteoarthritis, Springer Verlag. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Hashima C.Hamanishi S.Tanaka: "Muscarinic receptor-mediated Ca++control, protein kinase C activity and proteoglycan release and synthesis in articular chondrocytes" Advance in Osteoarthritis, Springer Verlag. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Satsuma H, Hamanishi C, Hashima M, Tanaka S,: "Alpha and epsilon isozymes of protein kinase C in the chondrocytes in normal and early osteoarthritic articular cartilage" Excerp.Med.32. 256 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Miyazaki H, Hamanishi C, Yoshii T, Tanaka S,: "Effect of a single intravenous dose of bisphosphonate(pamidronate)on tibial cortical bone after lengthening" Acta Medica Kinki Univ.22(2). 155-158 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Miyagi I, Kikuchi H, Hamanishi C, Tanaka S: "Auto-destruction of the articular cartilage and free radical mediators" J.Lab.Clin.Med. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Satsuma C.Hamanishi M.Hashima S.Tanaka: "Immunohistochemical localization of cartilage-specific and non-specific proteoglycans in experimental osteoarthritic articular cartilage in rats" Advance in Osteoarthritis,Springer Verlag. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] C.Hamanishi H.Miyazaki H.Satsuma S.Tanaka: "Protein kinase C-activator inhibits progression of osteoarthritis by inhibiting c-Fos protein expression in articular chondrocytes" Advance in Osteoarthritis,Springer Verlag. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Hashima C.Hamanishi S.Tanaka: "Muscarinic receptor-mediated Ca++ control,protein kinase C activity and proteoglycan release and synthesis in articular chondrocytes" Advance in Osteoarthritis,Springer Verlag. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Satsuma H,Hamanishi C,Hashima M,Tanaka S,: "Alpha and epsilon isozymes of protein kinase C in the chondrocytes in normal and early osteoarthritic articular cartilage" Calcified Tissue International,. 58:. 192-194 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hamanishi C,Hashima M,Satsuma H,Tanaka S: "Protein kinase C activator inhibited progression of osteoarthritis induced in rabbit knee joints" Journal of Laboratory and Clinical Medicine,. 127:. 540-544 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 戸谷由樹ほか: "ラットを用いた延長仮骨の免疫組織化学的検索" 中部日本整形外科災害外科学会誌. 38. 1197-1198 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 濱西千秋ほか: "創外固定を用いた骨折治癒と骨形成刺激の可能性" 臨床雑誌整形外科. 47. 357-363 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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