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Detection of unique gene in Hypertrophic scar (Keloid) fibroblast by mRNA differential display strangy.

Research Project

Project/Area Number 07671632
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKAWASAKI MEDICAL SCHOOL

Principal Investigator

MORIGUCHI Takahiko  KAWASAKI MEDICAL SCHOOL Plastic and Reconstructive Surgery, professor, 医学部, 教授 (10111816)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsdifferential display / hypertrophic scar / Keloid / fibroblasts / Differential Display
Research Abstract

Hypertrophic scar (Keloid) can form as a result of deep dermal injury, such as extensive thermal injury, and are characterized by an accumulation of the extracellular matrix in dermis. Lesional skin fibroblasts have been belived to play a crucial role in the disease development. Indeed, abnormal expresions of several extracellular matrix gene in lesional fibroblasts have been observed, however, the alternation mechinery of its function is still unclear. To address molecular mechanisms of change in fibroblasts, we employed a mRNA of differential display which is a novel gene subtraction strategy. Total RNA of cultured dermal fibroblasts derived from involved and uninvolved lesions of Hypertrophic scar patients (n=3) were extracted. Poly-A RNAs were converted into cDNA,amplified by a polymerase chain reaction using 20 sets of primers, then analyzed on a sequencing gel.
37 altered clones were detected in involved fibroblasts as compared to uninvolve fibroblasts. 4 clones of them were then sequenced.
To analyze details of these genes, full-length cloning and tissue in situ hybridization will be pursued. This approach may lead us to identify gene (s) associated with the nature of the disease.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 森口隆彦: "人工被覆材の現況と将来" 日本災害医学会誌. 44. 301-306 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 森口隆彦: "重度皮膚欠損創に対する真皮欠損用グラフト・ペルナックの臨床評価" 薬理と治療. 24. 153-171 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 森口隆彦: "熱傷瘢痕の予防" 救急医学. 20. 106-107 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 森口隆彦: "創傷被覆材の選択" 川崎医学会誌. 21. 287-296 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 森口隆彦: "創傷治癒.TEXT形成外科学" 南山堂, 11 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Moriguchi, Takahiko: "The current and future of the biological dressing" JJTOM. 44. 301-306 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Moriguchi, Takahiko: "Prevention of hypertrophic scar and contracture" J.J.Acute Medicine. 20. 106-107 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Moriguchi, Takahiko: "Select of Biologycal Dressing" Kawasaki MED.J.21. 287-296 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 森口隆彦: "人工被覆材の現況と将来" 日本災害医学会誌. 44. 301-306 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 森口隆彦: "熱傷瘢痕の予防" 救急医学. 20. 106-107 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 森口隆彦: "重度皮膚欠損創に対する真皮欠損用グラフト・ペルナックの臨床評価21GC03:薬理と治療" 24. 153-171 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 森口隆彦: "創傷被覆材の選択" 川崎医学会誌. 21. 287-296 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 森口隆彦: "創傷治癒.TEXT形成外科学" 南山堂, 11 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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