| Project/Area Number |
07671634
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | FUKUOKA UNIVERSITY |
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Principal Investigator |
OHJIMI Hiroyuki Fukuoka Univ.Hospital, Associate Professor., 助教授 (60160488)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | apoptosis / limb / ischemia / reperfusion injury / 再灌流障害 / Apotosis / No reflow phenomenon / 虚血再潅流障害 / 四肢 |
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| Research Abstract |
The mechanisms of cell death have been subject to debate. We studied whether an apoptotic type of cell death occurs in skin after ischemia and reperfusion injury.
METHODS AND RESULTS : We studied skin samples of rabbit lower limb that underwent ischemic condition achieved by 8 hours followed by 180 minutes of reperfusion. Extensive formation of DNA strand breaks, the typical biochemical feature of apoptosis was detected with the use of the in situ DNA end-labeling method. Apoptotic cells were observed particularly in the border zones of histologically necrotic skin, whereas very few apoptotic cells were present in the remote no necrotic skin.
CONCLUSIONS : This study provides evidence that in addition to overt necrosis, a subset of skin undergo apoptosis during ischemia-reperfusion injury. Apoptosis may provide a new target for skin slough during evolving ischemia and repurfusion injury in humans.
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