| Project/Area Number |
07671641
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Akita University |
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Principal Investigator |
MASAKI Yoko Akita Univ., School of Medicine, Instructor, 医学部, 助手 (30125744)
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| Co-Investigator(Kenkyū-buntansha) |
KADOSAKI Mamoru Akita Univ., School of Medicine, Instructor, 医学部, 助手 (10292369)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | T cell LAK-activity / Isoflurane / Halothane / Sevoflurane |
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| Research Abstract |
There have been many studies about immunological defects in patients undergoing general anesthesia and/or sugery in these 20years. However, it is not clear whether or not the volatile anesthetics have any effects on T cell functions. The aim of this study was to investigate the effect of volatile anesthetics on T cell-killer function.
Spleen cells from C57BL/6J mice preinjected anti-acialo GM1to deplete NKcell activity were harvested and resuspended at 1 * 10^6 cells/ml. After 96hr incubationwith with recombinantIL-2 (1000U/ml), cells were established as lymphokine activated killer T cells (T cell-LAK). The cell suspensions in 96 well plates were placed in the air tight acrylic chambers and exposed to the volatile anesthetics (Isoflurane, Halothane, Sevoflurane) containing air and 5% CO_2 at 37ﾟC.After 3hr exposure to anesthetics at each concentrations (0MAC,1MAC,2MAC,4MAC), cytotoxic killer activity was determined by^<51>Cr release assay. Furthermore, we investigated the effect of volatile anesthetics on the T cell-LAK development procedure by incucubation for initial 24hr or 96hr with these aneshtetics.
Volatile anesthetics inhibited T cell-LAK activity by IL-2. Isoflurane and Halothane inhibited significantly in a dose dependent fasion, but the inhibition by Sevoflurane was not significant. Isoflurane, Halothane and Sevoflurane also inhibited the development to T cell-LAK.Isoflurane and Halothane abolished T cell-LAK activity by 96hr incubotion. Sevoflurane also inhibited the development significantly but did not abolish the LAK activity. Their inhibition on the development to T cell-LAK was not completed by 24hr exposure.
We concluded that volatile anesthetics (Isoflurane, Halothane, Sevoflurane) inhibit not only T cell-LAK activity but also the development to T cell-LAK.
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