• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effects of volatile anesthetics on T cell functions

Research Project

Project/Area Number 07671641
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionAkita University

Principal Investigator

MASAKI Yoko  Akita Univ., School of Medicine, Instructor, 医学部, 助手 (30125744)

Co-Investigator(Kenkyū-buntansha) KADOSAKI Mamoru  Akita Univ., School of Medicine, Instructor, 医学部, 助手 (10292369)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsT cell LAK-activity / Isoflurane / Halothane / Sevoflurane
Research Abstract

There have been many studies about immunological defects in patients undergoing general anesthesia and/or sugery in these 20years. However, it is not clear whether or not the volatile anesthetics have any effects on T cell functions. The aim of this study was to investigate the effect of volatile anesthetics on T cell-killer function.
Spleen cells from C57BL/6J mice preinjected anti-acialo GM1to deplete NKcell activity were harvested and resuspended at 1 * 10^6 cells/ml. After 96hr incubationwith with recombinantIL-2 (1000U/ml), cells were established as lymphokine activated killer T cells (T cell-LAK). The cell suspensions in 96 well plates were placed in the air tight acrylic chambers and exposed to the volatile anesthetics (Isoflurane, Halothane, Sevoflurane) containing air and 5% CO_2 at 37゚C.After 3hr exposure to anesthetics at each concentrations (0MAC,1MAC,2MAC,4MAC), cytotoxic killer activity was determined by^<51>Cr release assay. Furthermore, we investigated the effect of volatile anesthetics on the T cell-LAK development procedure by incucubation for initial 24hr or 96hr with these aneshtetics.
Volatile anesthetics inhibited T cell-LAK activity by IL-2. Isoflurane and Halothane inhibited significantly in a dose dependent fasion, but the inhibition by Sevoflurane was not significant. Isoflurane, Halothane and Sevoflurane also inhibited the development to T cell-LAK.Isoflurane and Halothane abolished T cell-LAK activity by 96hr incubotion. Sevoflurane also inhibited the development significantly but did not abolish the LAK activity. Their inhibition on the development to T cell-LAK was not completed by 24hr exposure.
We concluded that volatile anesthetics (Isoflurane, Halothane, Sevoflurane) inhibit not only T cell-LAK activity but also the development to T cell-LAK.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Cullen BF,van Belle G: "Lymphocyte transformation and changes in leukocyte count : effects of anesthesia and operation." Anesthesiology. 43. 563-9 (1975)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Griffith C.D.M., Kamath M.B.: "Effect of halothane and nitrous oxide anesthesia on natural killer lymphocytes from patients with benign and malignant brest disease." Br J Anaeth. 58. 540-543 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mitsuhata H., Shimizu R.: "Supressive effects of volatile anesthetics on cytikine release in human peripheral blood mononuclear cells." Int J Immunopharmacol. 17. 529-534 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi