Effects of volatile anesthetics on T cell functions

Research Project

Project/Area Number	07671641
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Anesthesiology/Resuscitation studies
Research Institution	Akita University
Principal Investigator	MASAKI Yoko Akita Univ., School of Medicine, Instructor, 医学部, 助手 (30125744)
Co-Investigator(Kenkyū-buntansha)	KADOSAKI Mamoru Akita Univ., School of Medicine, Instructor, 医学部, 助手 (10292369)
Project Period (FY)	1995 – 1997
Project Status	Completed (Fiscal Year 1997)
Budget Amount *help	¥2,100,000 (Direct Cost: ¥2,100,000) Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000) Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000) Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords	T cell LAK-activity / Isoflurane / Halothane / Sevoflurane
Research Abstract	There have been many studies about immunological defects in patients undergoing general anesthesia and/or sugery in these 20years. However, it is not clear whether or not the volatile anesthetics have any effects on T cell functions. The aim of this study was to investigate the effect of volatile anesthetics on T cell-killer function. Spleen cells from C57BL/6J mice preinjected anti-acialo GM1to deplete NKcell activity were harvested and resuspended at 1 * 10^6 cells/ml. After 96hr incubationwith with recombinantIL-2 (1000U/ml), cells were established as lymphokine activated killer T cells (T cell-LAK). The cell suspensions in 96 well plates were placed in the air tight acrylic chambers and exposed to the volatile anesthetics (Isoflurane, Halothane, Sevoflurane) containing air and 5% CO_2 at 37ﾟC.After 3hr exposure to anesthetics at each concentrations (0MAC,1MAC,2MAC,4MAC), cytotoxic killer activity was determined by^<51>Cr release assay. Furthermore, we investigated the effect of volatile anesthetics on the T cell-LAK development procedure by incucubation for initial 24hr or 96hr with these aneshtetics. Volatile anesthetics inhibited T cell-LAK activity by IL-2. Isoflurane and Halothane inhibited significantly in a dose dependent fasion, but the inhibition by Sevoflurane was not significant. Isoflurane, Halothane and Sevoflurane also inhibited the development to T cell-LAK.Isoflurane and Halothane abolished T cell-LAK activity by 96hr incubotion. Sevoflurane also inhibited the development significantly but did not abolish the LAK activity. Their inhibition on the development to T cell-LAK was not completed by 24hr exposure. We concluded that volatile anesthetics (Isoflurane, Halothane, Sevoflurane) inhibit not only T cell-LAK activity but also the development to T cell-LAK.