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RESEARCH FOR MCP IN SEMEN AND SPERM-EGG INTERACTION

Research Project

Project/Area Number 07671721
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionOsaka University

Principal Investigator

MATSUMIYA Kiyomi  Osaka University Medical School, Instructor, 医学部, 講師 (90243237)

Co-Investigator(Kenkyū-buntansha) KITAMURA Masaya  Osaka University Medical School, Assistant Professor, 医学部, 助手 (70273688)
OKUYAMA Akihiko  Osaka University Medical School, Professor, 医学部, 教授 (20093388)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordssperm / MCP / CD46 / sperm-egg interaction / complement regulatory activity / monoclonal antibody
Research Abstract

1.Membrane cofactor protein (MCP), CD46, whose primary function is to protect host cells from homologous complement, has been presumed to serve as a sperm adhesion molecule for oocytes. The purpose of this study was to clarify the relationship between the properties of MCP expressed on epididymal sperm and their fertilizing ability in a recently developed strategy for assisted reproduction. We collected ejaculated sperm from normal subjects and epididvmal sperm from vasectomized subjects and patients with congenital absence of the vas deferens. Westent blotting and cofactor activity assay were performed to investigated the structural and functional properties of MCP.Epididymal spermatozoa which showed a reduced fertilizing ability tended to react poorly with antibodies against MCP and also showed low cofactor activity, indicating weak complement regulatory activity compared to that of ejaculated spermatozoa. MCP is sufficiently expressed in ejaculated sperm in men with a normally developed epididymis but is diminished in epididymal sperm from men with congenital or acquired obstruction of the vas deferens.
2.Three infertile subjects fulfillling normal or subnormal criteria on routine semen analysis showed abnormal sperm CD46 (membrane cofactor protein of complement) by SDS-PAGE/immunoblotting analysis using a panel of monoclonal antibodies. The sperm CD46 isoform has been reported to be associated with sperm-egg interaction. These 3 patients expressed normal CD46 isoforms on their lymphocytes and granulocytes. Thus, sperm-specific abnormalities in these proteins parallels male infertility, suggesting a new category of infertility, probably due to aberrations in the molecules related to sperm-egg interaction.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] M. Kitamura, K. Matsumiya et al.: "The fertilizing ability of human epididymal speum" Journal of Assisted Reproduction and Genetics. 13(8). 652-656 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M. Kitamura, K. Matsumiya et al.: "Possible association of in fertility with sperm-speific abnoumality of CD 46" Journal of Reproductive Immunology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitamura M,Matsumiya K,Namiki M,Hara T,Seya T and Okuyama A: "The Fertilizing Ability of Human Epididymal Sperm" Journal of Assisted Reproduction and Genetics. Volume 13, No.8. 652-656 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitamura M,and Seya T: "Function and clinical aspects of complement receptors on sperm" Clinical Immunology. Volume 28, No.4. 451-455 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitamura M,Matsumiya K,Yamanaka M,Takahara S,Hara T,Matsumoto M,Namiki M,Okuyama A and Seya T: "Possible Association of Infertility with Sperm-specific Abnormality of CD46" Journal of Reproductive Immunology. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M,Kitamura,K,Matsumiya et al.: "The fertilizing alility of human epididymal spernr" J.Assisted Reproduction and Genetics. 13(8). 652-656 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M,Kitamura,K,Matsumiya et al.: "Possible association of infertility with aperni-specific abnomality of CD46" Journal of Peproductive Immunology. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] 北村雅哉.瀬谷司: "精子に発現される補体関連分子群の機能とその臨床的意義" 臨床免疫. 28(4). 451-455 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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