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GENETIC ALTERATIONS OF PROSTATIC CANCER RELATING TO BONE METASTASIS AND PHENOTYPIC CHANGES.

Research Project

Project/Area Number 07671722
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionOsaka University

Principal Investigator

NONOMURA Norio  Osaka University Medical School, Assistant Professor, 医学部, 助手 (30263263)

Co-Investigator(Kenkyū-buntansha) KOJIMA Yasuyuki  Osaka University Medical School, Assistant Professor, 医学部, 助手 (50273628)
MIKI Tsuneharu  Osaka University Medical School, Associate Professor, 医学部, 助教授 (10243239)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsprostatic cancer / metastasis / genetic alterations / urological malignancies / testicular tumors / renal cell carcinoma / apoptosis / imprinting / 形質転換 / 遺伝子
Research Abstract

To begin with, we have done the clinical study of the prostatic cancer in our institute and reported the results. Because of small number of cases with low stage prostatic cancer, we could not examine thoroughly the expression of c-myc or c-fos oncogene or mutation of bcl-2 gene in the resected specimen. AT6.3 is a highly metastatic rat prostatic cancer cell line. We have reported the inhibitory effects of TNP-470, an angiogenesis inhibitor, on the lung metastasis of AT6.3 cells. We also found that human prostatic cancer cell lines, LNCap, DU145 and PC3 secrete an inhibitory activity on osteoblast-differentiation. The project of purifying this activity is now going on. Because we could not obtain materials from many cases of prostatic cancer, we examined the same kind of genetic alterations in other urological tumors such as renal cell carcinoma and testicular tumors which we often find at our institute. Genomic imprinting is a new entity of genetic regulation, in which certain genes such as insulin-like growth factor II (IGF2) and H19 genes are expressed only from unilateral allele. We found that IGF2 and H19 genes are very frequently expressed from unilateral allele in testicular cancers. This genetic alteration is now under investigation on prostatic cancer. Apoptosis is a new concept of cell death, and it is typically induced through Fas-Fas ligand pathway in many cells. Prostatic cancer cells do not show the typical apoptosis through the stimulation with Fas-pathway. On the contrary, renal cell carcinoma express Fas antigen, and showed typical Fas-mediated apoptosis, which was enhanced by incubating in the presence of interferon-g. All these examinations can be applied to prostatic cancer.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 児島 康行・近藤 雅彦,野々村 祝夫ら: "前立腺癌の臨床統計的検討" 西日本泌尿器科. 第8巻2号. 115-119 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N. Nonomura et al.: "Fas/APO-1-mcdiated apoptosis of human reual cell cascinoma" Biochomical and biophysical research aommunications. 229. 945-951 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N. Nonomura et al.: "Altercd imprinting of the H19 and insulin-like grmth factor II genes in testiculan tumors" Journal of Ueology. 157(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kojima Y,Kondo M,Nonomura N,Kondo M,Seguchi T,Miki T,Nakano E,Okuyama A and Kuroda H: "Clinical Survey of Prostate Cancer." Nishinihon Journal of Urology. Volume 58, No.2. 115-119 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nonomura N,Miki T,Nishimura K,Kanno N,Kojima Y and Okuyma A: "Altered Imprinting of the H19 and Insulin-like Growth Factor II Genes in Testicular Tumors." Journal of Urology. Volume 157 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nonomura N,Miki T,Yokoyama M,Imazu T,Takada T,Takeuchi S,Kanno N,Nishimura K,Kojima Y and Okuyama A: "Fas/APO-1-Mediated Apoptosis of Human Renal Cell Carcinoma" Biochemical and Biophysical Research Communications. Volume 229. 945-951 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 児島康行・近藤雅彦 野々村 祝夫 ら: "前立腺癌の臨床統計的検討" 西日本泌尿器科. 第58巻・2号. 115-119 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Norio Nonomura et al.: "Fas/APO-I-mediated apoptosis of human renal cell carcinoma" Biochemical and biophysical research communications. 229. 945-951 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Norio Nonomura et al.: "Altered imprinting of the H19 and insulin-like growth factor II genes in testicular tumors" Journal of Urology. 157(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 児島康行: "前立腺癌の臨床統計学検討" 西日本泌尿器科. 58. 115-119 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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