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Cellular and Molecular Biological Study on Invasion and Metastasis of Urogenital Cancer

Research Project

Project/Area Number 07671741
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionSapporo Medical University School of Medicine

Principal Investigator

MIYAO Noriomi  Sapporo Medical University School of Medicine Assistant Professor, 医学部, 講師 (40200125)

Co-Investigator(Kenkyū-buntansha) YANASE Masahiro  Sapporo Medical University School of Medicine Clinical Instructor, 医学部, 助手 (80291558)
TSUKAMOTO Taiji  Sapporo Medical University School of Medicine Professor, 医学部, 教授 (50112454)
岩澤 晶彦  札幌医科大学, 医学部, 助手
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
KeywordsProstate cancer / Invasion / Metastasis / Extracellular matrix degradation / Urokinase type-plasminogen activator / transcription factor / 浸潤・転移 / 遺伝子導入 / 浸潤能 / 浸潤抑制
Research Abstract

[Background] : Although many factors influence cancer cell invasion and metastasis formation, degradation of extracellular matrices has been reported to be a crucial step. The number of patient with prostate cancer has been increasing recent years in Japan. Radical prostatectomy has been reported the best treatment for organ confined prostate cancer, clinical outcomes of locally advanced and systemic spread diseases are miserable. The mechanisms of prostate cancer cell invasion and metastasis have not been fully understood. In this project we investigated that one of the mechanisms of prostate cancer invasion and metastasis.
[Results] : In vitro invasiveness of human prostate cancer cell lines, PC-3 and LNCaP,was determined by Matrigel in vitro invasion assay system indicating that PC-3 demonstrated significant in vitro invasiveness comparing to LNCaP at 6 and 8 hours incubations (p<0.01). PC-3 demonstrated to be strongly expressed E1AF and urokinase type-plasminogen activator (u-PA) mRNA,but not in LNCaP.Deletion mutant E1AF gene was introduced to highly invasive PC-3 cells by calcium-phosphate method. u-PA zymogram revealed that PC-3 degrade u-PA and that u-PA activity on 33kD was reduced in transfectants of deletion mutant E1AF comparded to that of transfectants of empty vecor to PC-3. PC-3 transfectants of deletion mutant E1AF expressed wild type E1AF and deletion mutant E1AF by Northern blot analysis. u-PA expression was reduced in transfectants of deletion mutant E1AF but no to be reduced wild type E1AF.In vitro invasiveness of deletion mutant E1AF tranfectants was apparently suppressed in 8 hours incubation compared to that of transfectant of empty vector. CAT assay for promoter activity of u-PA gene also demonstrated the supportive results that E1AF activates trancription of u-PA gene.
[Conclusions] : Prostate cancer cell invasion was regulated by expression of u-PA and expression of u-PA is regulated by transcription factor, E1AF.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Miyao N, et al.: "Preoperatively determined prognostic factors in metastatic renal cell carcinoma." European Urology. 31. 292-296 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyao N, et al.: "Lymph node metastasis in the patients with carcinoma of renal pelvis and ureter." European Urology. 33. 180-185 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takahashi A, Miyao N, et al.: "Treatment for advanced testicular cancer with high-dose chemotherapy and autologous blood stem cell transplantation." International Journal of Urology. 5. 67-73 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oda T,Takahashi A,Miyano N,et al.: "Clinical evaluation of serum prostate specific antigen in the diagnosis of prostate cancer by chemiluminescence immunoassay system (ACS-PSA)." Jpn.J.Urol.Surgery. 8. 1031-1037 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hotta H,Miyano N,Masumori N,et al.: "A clinical study of radical prostatectomy." Jpn.J.Urol.87. 760-776 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitamura H,Masumori N,Takahashi A,Sasamura K,Oda T,Miyao N,et al: "Clinical study of 202 patients with prostate carcinomo." Jpn.Clin.Urol.50. 404-409 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takahashi S,Sakai S,Koroku M,Sano M,Shimamura S,Miyao N,et al.: "Renal cell carcinoma in young adults." Jpn.J.Urol. Surgery. 9. 837-840 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyao N,Oda T,Shigyou M,et al.: "Preoperatively determined prognostic factors in metastatic renal cell carcinoma." Eur.Urol.31. 292-296 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miyao N,Masumori N,Takahashi A,et al.: "Lymph node metastasis in the patients with carcinoma of renal pelvis and ureter." Eur.Urol.33. 180-185 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takahashi A,Miyao N,Masumori N,et al.: "Treatment for advanced testicular cancer with high-dose chemotherapy and autologous blood stem cell transplantation." Int.J.Urol.5. 67-73 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 塚本泰司、柳瀬雅裕: "偶然発見される腎細胞癌 -最近の動向-" 「最新 腎移植と腎癌手術-可能性と問題点-」田島惇編集、協和企画. 85-90 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Miyao N,Oda T,et al.: "Pre-operatively determined prognostic factor in metastatic renal cell carcinoma" European Urology. 31. 292-296 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Miyao N,Masumori N,et al.: "Lymph node metastasis in patients with carcinomas of the renal pelyis and ureter" European Urology. 33. 180-185 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masumori N,et al.: "Inhibitory effect of minocycline on in vitro invasion and experimental metastasis of mouse renal adenocarcinoma" Journal of Urology. 151. 1400-1404 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] 尾田寿朗、他: "前立腺癌診断における血清PSAの検討-Chemoiluminescence immunoassay PSA (ACS-PSA)-" 泌尿器外科. 8. 1031-1037 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 堀田 裕、他: "根治的前立腺摘除術の臨床的検討" 日本泌尿器科学会誌. 87. 760-765 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北村 寛、他: "前立腺癌202例の治療成績の検討" 泌尿器科紀要. 50. 404-409 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yanase M.: "Cytokines modulate in vitro invasiveness of renal cell carcinoma cells through action on the process of cell attachment to endothelial cells" Journal of Urology. 153. 844-848 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masumori N.: "Interferon-Gamma and interleukin-2 suppress the experimental metastasis of mouse renal adenocarcinoma" International Journal of Urology. 2. 6-11 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 堀田 裕、宮尾則臣、他: "根治的前立腺摘除術の臨床的検討" 日本泌尿器科学会雑誌. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] 北村 寛、宮尾則臣、他: "当科における前立腺癌202例の治療方法および治療成績の検討" 臨床泌尿器科. (印刷中).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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