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Effect of donor specific antigen on graft survival

Research Project

Project/Area Number 07671752
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionKeio University

Principal Investigator

MURAI Masaru  Keio Univ.School of Med.Professor, 医学部, 教授 (90101956)

Co-Investigator(Kenkyū-buntansha) SAITO Shiro  Keio Univ.School of Med.Instructor, 医学部, 助手 (80170504)
TACHIBANA Masaaki  Keio Univ.School of Med.Assistant Prof., 医学部, 講師 (70129526)
BABA Shiro  Keio Univ.School of Med.Associate Prof., 医学部, 助教授 (00051889)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsrenal transplantation / immunosuppression / lymphocyte subsets / DST / 免疫抑制療法
Research Abstract

Donor-specific transfusion (DST) had once been widely accepted to improve living-related renal allograft survival. Previous studies have reported that transfusion can induce suppressor cell and/or anti-idiotypic antibodies. The aim of this study was to clarify the mechanism of effect of DST on renal allograft survival.
The prospective kidney recipient received transfusion of the respective donors whole blood of 100ml at 1,8 and 15 days after its storage. During DST,patients received cyclosporine to minimize sensitization. Peripheral blood lymphocytes were analyzed by flowcytometer for the presence of surface antigenes using monoclonal antibodies.
The percentage of CD8^+ cells rose significantly 1 week after the initial DST and was sustained throughout the remainder of the study, leading to a decreased CD4^+/CD8^+ cells ratio. The percentage of HLA-DR^+ cells also increased after DST.These results differ partly form those of Lenhard et al who studied the effect of blood transfusion on suppressor cells in hemodialysis patients. They showed an increased percentage of HLA-DR^+ cells as we did, but a decrease in OKT4/OKT8 ratio, concluding that monocytes act as suppressor cells in the immediate posttransfusion period.
Increase in CD11b^+/CD8^+ cells in addition to increase in CD8^+ cells this study suggests participation of suppressor T cell in immunological effects of DST combined with CyA.To define whether or not DST combined with CyA still maintains immunologically beneficial effects, the MLR suppressor cell assay was performed, in which three of six patients were positive.
The present study is preliminary because of the restricted number of patients studied. These results, however, suggest that DST combined with CyA can still induce suppressor cells while minimizing the sensitization.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Masaru Murai: "Effect of DST combined with cycloporine on lymphocyte subsets of potential recipients for living related donor allograft." Geriatric Medicine. 26. 1950-1951 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 村井 勝: "高齢者と腎移植" Geriatric Medicine. 34・11. 1513-1515 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 村井 勝: "高尿酸血症(腎移植患者の管理)" 腎と透析. 41(Supple). 675-676 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Murai, H.Nakamura, S.Sekiguchi, S.Shimizu and F.Nakajima: "Effect of DST combined with cyclosporine on lymphocyte subsets of potential recipients for living-related donor renal allograft." Transplantation Proceedings. 26. 1950-1951 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Murai: "Renal transplantation in high aged recipient or donor." Geriatric Medicine. 34. 1513-1515 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Murai: "Hyperuricemia in renal transplantation." Kidny and Dialysis. 41 (supple). 675-676 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 村井 勝: "高齢者と腎移植" Geriatric Medicine. 34・11. 1513-1515 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 村井 勝: "高尿酸血症(腎移植患者の管理)" 腎と透析. 41(Supple). 675-676 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masaru Murai: "Effect of DST combined with cyclosporine on lymphocyte subsets of potential recipients for living related donor allograft." Transplantation Proceedings. 26. 1950-1951 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 村井 勝: "系統看護学講座" 医学書院, 275 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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