| Project/Area Number |
07671759
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | KAWASAKI MEDICAL SCHOOL |
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Principal Investigator |
MATSUMOTO Akira Kawasaki Medical School, School of Medicine, Professor, 医学部, 教授 (90027318)
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| Co-Investigator(Kenkyū-buntansha) |
BESSHO Hiroko Kawasaki Medical School, School of Medicine, Assistant, 医学部, 助手 (10104811)
YAMADA Sakuo Kawasaki Medical School, School of Medicine, Instructor, 医学部, 講師 (00122458)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | surface projection / Chlamydia trachomatis / elementary body / Sarkosyl extraction / 36KDaサブユニット / セアクリルゲル濾過 / Chlamydia trachomatis / Chlamydia psittaci / Chlamydia pneumoniae |
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| Research Abstract |
To study the function of surface projections of C.trachomatis and C.psittaci organisms, we examined following three points ; (1) establishment of an efficient method for mass-production of projections, (2) protein composition of isolated projections and (3) effect of anti-projection antibody on the chlamydial infectivity. The results are summarized as follows ; (1) the projections were efficiently extracted from envelopes of elementary bodies (EBs) through a newly designed procedures including Sarkosyl treatment and seacryl S-1000 gel-filtration without any damage to the morphology of projections. (2) the silver staining after PAGE showed two miner bands (30 and 32 KDa) and one major band (36KDa), although no band was detected by the Coomassie brilliant brilliant blue staining. From the results of electron microscopic observations and PAGE,the stem part of projection appeared to be composed of 36KDa protein subunits. (3) Rabbit sera hyperimmunized with intact EBs were reactive with isolated projections after adsorption with EB outer membranes and showed neutralizing activity to the EB infectivity. However, sera prepared with 36KDa protein, which was transferred onto a cellulose membrane and then was implanted subcutaneously, did not show any reacivity with isolated projections, indicating insufficient quantity of 36KDa protein for antibody production.
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