ELUCIDATION OF CARCINOGENESIS OF HUMAN PAPILLOMAVIRUS (HPV) NEGATIVE CERVICAL LESIONS-PARTICIPATION WITH p53 MUTATIONS-
| Project/Area Number |
07671767
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | CHIBA UNIVERSITY |
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Principal Investigator |
NUNOYAMA Takafumi CHIBA UNIVERSITY SCHOOL OF MEDICINE,ASSISTANT, 医学部, 助手 (90228271)
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| Co-Investigator(Kenkyū-buntansha) |
ONODERA Tsutomu CHIBA UNIVERSITY HOSPITAL,SENIOR RESIDENT, 医学部・附属病院, 医員
SEKIYA Souei CHIBA UNIVERSITY SCHOOL OF MEDICINE,PROFESSOR, 医学部, 教授 (00092065)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | CARCINOGENESIS OF HUMAN CERVICAL CARCINOMAS / HUMAN PAPILLOMAVIRUS / TUMOR SUPPRESSOR GENE / MUTATION OF p53 GENE / p53遺伝子変異 |
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| Research Abstract |
To evaluate the clinical significance of human papillomavirus (HPV) infection for the carcinogenesis of human cervical carcinomas, we have documented the presence of HPV-DNA in biopsied specimens from 131 cervical lesions (94 cervical dysplasias and 37 cervical carcinomas). Each samples was screened by low-stringency Southern blot hybridization and polymerase chain reaction (PCR) with the consensus primer for L1 region. HPV-DNA was detected in 81% of cervical dysplasias and in 70% of cervical carcinomas. The types of infecting HPV were as follows ; High risk HPV (type 16/18) (25%), other known types (42%), unknown types (11%).
It is reported that mutation of tumor-suppressor gene p53 participates with carcinogenesis of HPV-negative cervical carcinoma cell lines. Human cervical carcinoma cell lines were analyzed for evidence of mutation of the p53 gene using PCR single-strand conformation polymorphism (SSCP). Each of seven HPV-positive cervical carcinoma cell lines and HPV-negative cervical carcinoma cell line (YUMOTO) demonstrated wild-type p53.
The clinical and pathological significance of mutation of p53 was examined in 36 cervical lesions (9 cervical dysplasias and 27 cervical carcinomas) using PCR-SSCP.Mutations of the p53 gene was detected in only one HPV-negative cervical carcinoma of 36 cervical lesions. These results suggest that the inactivation of the tumor-suppressor gene p53 is not important step in human cervical carcinogenesis.
Clinical follow-up study of 24 cervical dysplasias were examined. The follow-up time was 6-15 months. Sixteen (76%) women showed regressive, 4 (19%) women showed persistent, and one (5%) woman showed progressive.
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Report
(3 results)
Research Products
(4 results)
