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A genetherapy for the cervical cancer based on its carcinogenesis.

Research Project

Project/Area Number 07671804
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionSaga Medical School

Principal Investigator

SUGIMORI Hajime  Saga Medical School Dept.Medecine Professor, 医学部, 教授 (50038642)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Satoru  Saga Medical School Dept.Medicine Assistant, 医学部, 助手 (30217848)
YOKOYAMA Masatoshi  Saga Medical School Dept.Medicine Assistant, 医学部, 助手 (40230669)
FUKUDA Kouichi  Saga Medical School Dept.Medicine Lecturer, 医学部, 講師 (80189943)
IWASAKA Tsuyoshi  Saga Medical School Dept.Medicine Assit.Prof., 医学部, 助教授 (60117067)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
KeywordsCervical cancer / p53 / genetherapy / p16 / HPV / 免疫染色
Research Abstract

1.Tne primary culture of squamous epithelial cells and columnar cells from the normal human uteirne cervix was performed. HPV 16 or 18 was transfected to these cells and the imortalized cell lines were established. Furthermore, the malignant transformation was induced by transfection of the carcinogens. Thus, the in-vitro model of multi-step carcinogenesis of the cervical cancer was completed.
2.P16, cyclin dependent kinase inhibitor, was examined concerning deletion or point mutation. Although p16 was overexpressed in immortalized cell lines, its deletion was not detected in these in-vitro model. P16 was supposed to be not so important in carcinogenesis of the cervical ancer.
3.PMO-hp53 is plasmid that induce wild type p53 by dexamethasone. PMO-hp 53 was transfected to the cervical cancer cell line, TMCC-1 and ME180. By this transfection The cell line showed the following changes.
(1) Cell cycle was stopped in M-phase and cell proliferation was disturbed.
(2) Cell enlatrgment, multi-nucleation, micro-nucleation and changes in cytoplasm were observed.
(3) Proliferation activity in soft agar was dismissed.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] Mvula M: "Human papillomavirus DNA sequences in cervical carcinomas of Zairean women." The Cervix & l.f.g.t.13. 91-94 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwasaka T: "Outpatient treatment of cervical intra-epithelial neoplasia with laser conization." The Cervix & l.f.g.t.13. 99-103 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hara K: "Effects of single and combined application of anti-cancer drugs on cervical adenocarcinoma.I.Antitumor activity in vitro." Acta Obstet Gynecol Scand. 74. 330-335 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zheng PS: "Simultaneous detection by consensus multiplex PCR of high-and low-risk and other types of human papillomavirus in clinical samples." Gynecol Oncol. 58. 179-183 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwai K: "Immunohistochemical detection of p53 overexpression in formalin-fixed,paraffin-embedded sections of endometrial carcinoma." J Obstet Gynecol. 21. 593-600 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwasaka T: "Non-detection of human papillomavirus DNA in cervical dysplasia and disease progression." Lancet. 348. 333-334 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zheng PS: "Growth suppression of a cervical cancer cell line(TMCC-1)by the human wild-type p53 gene." Gynecol Oncol. 60. 245-250 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mvula M: "Do human papillomavirus have a role in the pathogenesis of bladder carcinoma?" J Urol. 155. 471-474 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hachisuga T: "Human papillomavirus and p53 gene overexpression in carcinoma of the uterine cervix,lower uterine segment and endometrium." Pathology. 28. 28-31 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwasaka T: "Cytologic changes in two cervical carcinoma cell lines after transfection of the wild-type p53 gene." Acta Obstet Gynecol Scand. 75. 797-803 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岩坂 剛: "HPV陽性女性の追跡." 産婦の実際. 45. 311-317 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岩坂 剛: "p53遺伝子導入による子宮頚癌治療の試み." 産婦治療. 72. 994-994 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zheng PS: "Telomerase activity in gynecologic tumors." Gynecol Oncol. 64. 171-175 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uchiyama M: "Correlation between human papilloma virus positivity and p53 gene overexpression in adenocarcinoma of the uterine cervix." Gynecol Oncol. 65. 23-29 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zheng PS: "Telomerase activation in invitro and in vivo cervical carcinogenesis." Gynecol Onol. 66. 222-226 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mvula M: "Human papillomavirus DNA sequences in cervical carcinomas of Zairean women" The Cervix & l.f.g.t. 13. 91-94 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwasaka T: "Outpatient treatment of cervical intra-epithelial neoplasia with laser conization" The Cervix & l.f.g.t. 13. 99-103 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hara K: "Effects of single and combined application of anti-cancer drugs on cervical adenocarcinoma. I.Antitumor activity in vitro" Acta Obstet Gynecol Scand. 74. 330-335 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zheng PS: "Simultaneous detection by consensus multiplex PCR of high-and low-risk and other types of human papillomavirus in clinical samples" Cynecol Oncol. 58. 179-183 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwai K: "Immunohistochemical detection of p53 overexpression in formalin-fixed, paraffin-embedded sections of endometrial carcinoma" J Obstet Gynecol. 21(6). 593-600 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwasaka T: "Non-detection of human papillomavirus DNA in cervical dysplasia and disease progeression" Lencet. 348. 333-334 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zheng PS: "Growth suppression of a cervical cancer cell line (TMCC-1) by the human wild-type p53 gene" Gynecol Oncol. 60. 245-250 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mvula M: "Do human papillomavirus have a role in the pathogenesis of bladder carcinoma?" J Urol. 155. 471-474 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hachisuga T: "Human papillomavirus and p53 gene overexpression in carcinoma of the uterine cervix, lower uterine segment and endometrium" Pathology. 28. 28-31 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwasaka T: "Follow up study of HPV positive women" Sanfu no Jissai. 45. 311-317 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zheng PS: "Telomerase activity in gynecologic tumors" Gynecol Oncol. 64. 171-175 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uchiyama M: "Correlation between human papilloma virus positivity and p53 gene overexpression in adenocarcinoma of the uterine cervix" Gynecol Oncol. 65. 23-29 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zheng PS: "Telomerase activation in invitro and in vivo cervical carcinogenesis" Gynecol Onol. 66. 222-226 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Zheng,Peng-Sheng: "Teromerase activity in gynecologic tumors." Gynecol Oncol.64.1. 171-175 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Uchiyama,Michiko: "Correlation between human papilloma virus positivity and p53 overexpression in adenocarcinoma." Gynecol Oncol. 65.1. 23-29 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Zheng,Peng-Sheng: "Teromerase activation in vitro and in vivo cervical carcinogenesis." Gynecol Oncol. 66.2. 222-226 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hachisuga,Toru: "Human papilloma virus and p53 expressionin carcinoma of the uterine cervix" patholgy. 28.1. 28-31 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Zheng,peng-sheng: "Growth suppression of a cervical cahcer cell line by the human wild-tupe p53 gene." Gynecol Oncol. 60.2. 245-250 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Iwasaki,Tsuyoshi: "Non-detection of human papilloma virus in cervical dysplasia." Lancet. 348.9023. 333-334 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Iwasaki,Tsuyoshi: "Cytologic changes in two cervical carcinoma cell line after transfection of the wild-type p53 gene." Acta Obstet Gynecol Scand. 75.9. 793-803 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岩坂剛: "p53遺伝子導入による子宮頚癌治療の試み" 産婦人科治療. 72.6. 994 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岩坂 剛: "子宮頚癌-ウイルス発癌機構" 臨床婦人科産科. 49. 441-447 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoyama,Masatosi: "Alterations in physical state and expression of human papilloma type 18 DNA." Virus Research. 37. 139-151 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mvula,Mansuka: "Human papilloma virus DNA sequences in cervical carcinomas of Zairean women." The Cervix and l.f.g.t.13. 91-94 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Iwai,Kyouko: "Immunohisochemical detection of p53 overexpression in formalin-fiex,paraffin-embedded sections." J.Obstet.Gynecol.21. 593-600 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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