| Project/Area Number |
07671807
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Miyazaki Medical College |
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Principal Investigator |
NAGAI Kimihiro Miyazaki Medical College, Perinatal Center, Assistant Professor, 医学部, 講師 (20207963)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | human breast milk / inhibitor of prostaglandin dehydrogenase / fatty acids / 壊死性腸炎 / 母乳 / ガスクロマトグラフィー / プロスタゲランディン / 脂質 |
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| Research Abstract |
The inhibitors of prostaglandin dehydrogenase were extracted from human breast milks to be purified and analyzed. The human breast milk was treated by acetone and petroleum ether followed by the evaporation and the resuspension in ethanol.
The inhibitors were further purified by high performance thin layer chromatography (HPTLC), was developed with the solvent system of chloroform : acetone (96 : 4, v/v). After the extraction from the silica plate with ethylether, the inhibitors were purified by HPTLC with the solvent system of petroleum ether : ethyl ether (80 : 20, v/v). The analysis of the inhibitors was carried out with gas chromatography mass spectrometry (GC/MS-QP2000A), equipped with a 25m*0.25mm cappillary URBON HR-101 column. The working conditions were as follows : injector and detector temperature 280ﾟC, helium as carrier gas, oven temperature programmation from 50ﾟC to 280ﾟC at 10ﾟC/ min. The inhibitors was found to consist of the mixture of nine fatty acids. The major peaks were three fatty acids : myristic acid, palmitic acid and stearic acid. Other acids were these dervivatives. The mixture of these fatty acids inhibited the activity of prostaglandin dehydrogenase in a dose - dependent manner in vitro.
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