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Immunological Function of HLA-G Exepressed on trophoblasts.

Research Project

Project/Area Number 07671815
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionNara Medical University

Principal Investigator

ISHITANI Akiko  Nara Medical University, Departement of Medicine, Assistant Research er, 医学部, 助手 (40112544)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsHLA-G / placenta / trophoblast / monoclonal antibody / immunohistochemistry / immunosupressor / reproductive immunology
Research Abstract

To understand the basic relationship of HLA class I to maternal-placental immune tolerance, we have attempted to dissect the expression of soluble (S) HLA-G from menbrance bound (M) HLA-G and to investigate the expression of other nonclassical class I,specifically HLA-E.Towards this goal, we have developed a set of HLA-G specific monoclonal antibodies which allow the distinction of the soluble and membrane G1 proteins, and have used these and specific anti-HLA-E reagents in immunohisto-chemical staining to localize these three proteins in placental tissue. Further, we used HLA-G specific antibodies to isolate S・HLA-G and M・HLA-G directly from placental tissue in order to examine bound peptides.
1. Expression of HLA-G and HLA-E
Immunohistochemical staining of placenta demonstrated that S・HLA-G was found expressed in syncyciotrophoblasts, cytotrophoblasts, and extravillous cytotrophoblasts and was also apparent in maternal blood at intervillous space. Consistent with previous reports, M・HLA-G was found expressed only in extravillous cytotrophoblasts. HLA-E was expressed weakly in synciciotrophoblasts and cytotrophoblasts and comparatively more strongly in extravillous cytotrophoblasts, essentially in parallel with total HLA-G.
2. Antigen presenting ability of HLA-G
To investigate the ability, TAP dependency of HLA-G expression, and sequence of HLA-G bound peptide were examined. When HLA-G was isolated from transfected cells, a relatively large number of bound peptides derived from intra cellular proteins were identified, but the number of bound peptides was considerably smaller and the individual abundance considerably higher, with a single peptide species accounting for 15% of the total peptide bound to HLA-G from the placenta.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 石谷昭子: "HLA-G発現によるNK細胞障害からの回避" 臨床免疫. (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 石谷昭子: "HLA-Gは抗原提示できるか" 臨床免疫. 29. 336-342 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ni Lee: "The membrane-bound and soluble forms of HLA-G bind identical sets of endogenous peptides but differ with respect to TAP association." Immunity. 3. 591-600 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiko Ishitani: "Localization of HLA-G protein" Placenta. 16. A4 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 石谷昭子: "胎盤におけるHLA-Gの役割" 臨床免疫. 27. 1367-1372 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 石谷昭子: "Alternative splicingにより産生される多様なHLA-G mRNAおよびそれら蛋白に対するモノクローナル抗体について" MHC. 2. 98 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiko Ishitani: "Protection from NK lysis by expression of HLA-G" Clinical Immunology. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiko Ishitani: "Antigen presentation by HLA-G" Clinical Immunology. 29-3. 336-342 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ni Lee: "The membrane-bound and soluble forms of HLA-G bind identical sets of endgenous peptides but differ with respect to TAP association" Immunity. 3. 591-600 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiko Ishitani: "Localization of HLA-G protein" Placenta. 16. A4 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiko Ishitani: "The role of HLA-G in placenta" Clinical Immunology. 27. 1367-1372 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akiko Ishitani: "A set of Monoclonal antibodies against alternatively spliced mRNAs and proteins of HLA-G" MHC. 2. 98 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 石谷昭子: "HLA-G発現によるNK細胞障害からの回避" 臨床免疫. 印刷中. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石谷昭子: "HLA-Gは抗原提示できるか" 臨床免疫. 29. 336-342 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Lee N.: "The membrane-bound and soluble forms of HLA-G bind identical sets of endogenous peptides but differ with respect to TAP association." Immunity.3. 591- 600 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishitani A.: "Localization of HLA-G protein" Placenta. 16. A4 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石谷昭子: "胎盤におけるHLA-Gの役割" 臨床免疫. 27. 1367-1372 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石谷昭子: "Alternative splicingにより産生される多様なHLA-G mRNAおよびそれら蛋白に対するモノクローナル抗体について" MHC. 2. 98 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ni Lee: "The membrane-bound and soluble forms of HLA-G bind identical sets of endogeneous peptides but differ withrespect to TAP association" Immunity. 3. 591-600 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Akiko Ishitani: "Localization of HLA-G protein" Placenta. 16. A4 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 石谷昭子: "胎盤におけるHLA-Gの役割" 臨床免疫. 27. 1367-1372 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 石谷昭子: "Alternative splicingにより産性される多様なHLA-G mRNAおよびそれら蛋白に対するモノクローナル抗体について" MHC. 2. 98 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 長池知恵子: "HLA-Gモノクローナル抗体を使用したヒト各種組織および各妊娠周期胎盤におけるHLA-G抗原の検索" MHC. 2. 99 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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