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Degradation system of extracellular matrix components in nasal polyp formation

Research Project

Project/Area Number 07671851
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionMie University

Principal Investigator

HARADA Teruhiko  Mie University, Hospital Lecturer, 医学部・附属病院, 講師 (20183569)

Co-Investigator(Kenkyū-buntansha) MAJIMA Yuichi  Mie University, Hospital Lecturer, 医学部・附属病院, 講師 (60024791)
SAKAKURA Yasuo  Mie University, Faculty of Medicine, Professor, 医学部, 教授 (40024723)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsNasal polyp / Paranasal sinusitis / Nasal allergy / Extracellular matrix / Matrix metalloproteinase
Research Abstract

The etiology and pathogenesis of nasal polyps and polypoid formations of nasal of paranasal mucosa have been the subjects of debate. We have reported that the extracellular matrix is a complex aggregate of distinct collagenous and non-collagenous protein components in nasal polyps, which in physiologic situations are in a dynamic equlibrium.
Remodeling of the extracellular matrix is a prominent feature in various biological processes ranging from wound healing and tissue development to tumor invasion and chronic inflammatory disorders. During nasal polyp formation, degradative enzymes are probably involved in the removal of devitalized tissues, epidermal : mesenchymal interactions, angiogenesis, and remodeling of newly synthesized connective tissue. We have, therefore, studied the distribution of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in nasal and paranasal mucosa, in an attempt to elucidate the mechanism of nasal polyp formation.
The distribution of 4 major MMPs was studied in nasal polyps : collagenase (MMP-1) which degrades fibrillar interstitial collagens, a 72-kDa gelatinase (MMP-2) which mainly degrades type IV collagen and denatured collagens, stromelysin (MMP-3) which has a wider range of action, and a 92-kDa gelatinase (MMP-9) which has a wide range of substrate specificity against collagens. These MMPs and the MMP tissual inhibitors (TIMP-1 and TIMP-2) were detected by immunohistochemistry. MMP-1 and MMP-2 were detected in the fibroblasts at the periphery fo nasal polyp stalks. MMP-9 was detected in the inflammatory cells in the subepithelial area of polypoid mucosa and nasal polyps. TIMP-1 and TIMP-2 were also detected in a few fibroblasts in the edematous mucosa. These results suggest the role of MMPs in the breakdown of collagenous and non-collagenous connective tissue and basement membranes. The disequilibrium of MMPs and TIMPs in nasal and paranasal mucosa may promote nasal polyp growth.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 野々山 勉、他: "慢性副鼻腔炎におけるサイトカインの免疫組織学的検討" 免疫アレルギー. 14. 180-181 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 原田 輝彦、他: "鼻茸形成における細胞外マトリックス分解機構の検討" 免疫アレルギー. 15. 40-41 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Guo Y, et al.: "Effects of functional endoscopic sinus surgery on maxillary sinus mucosa" Arch Otolaryngol Head Neck Surg. 123. 1097-1100 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Majima Y, et al.: "Quantitative study of nasal secretory cells in normal subjects and patients with chronic sinusitis" Laryngoscope. 107. 1515-1518 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada T, et al.: "The role of extracellular matrix and their degradation system in nasal polyp formation" Am J Rhinol. 11. 412-413 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shinogi J, et al.: "Quantitative cytology of nasal secretions with perennial allergic rhinitis in children:Comparison of non-infected and infected conditions" Laryngoscope. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 原田 輝彦(分担執筆): "細胞外マトリックス" 板倉照好編、羊土社、東京, 172(12) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nonoyama T., et al.: "Immunolocalization of cytokines in the paranasal mucosa in the patients with chronic sinusitis." Journal of Japan Society of Immunology & Allergology in Otolaryngology.14. 180-181 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada T,et al.: "Degradation system of extracellular matrices in nasal polyp formation." Journal of Japan Society of Immunology & Allergology in Otolaryngology.15. 40-41 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Guo Y,et al.: "Effects of functional endoscopic sinus surgery on maxillary sinus mucasa." Arch Otolaryngol Head Neck Surg.123. 1097-1100 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Majima Y,et al.: "Quantitative study of nasal secretory cells in normal subjects and patients with chronic sinusitis." Laryngoscope. 107. 1515-1518 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada T,et al.: "The role of extracellular matrix and their degradation system in nasal polyp formation." Am J Rhinol.11. 412-413 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Guo Y,et al.: "Cultured human nasal gland cells in a three-dimensional collagen gel." In Vitro Cell Biol-Animal. 34. 16-18 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shinogi J,et al.: "Quantitative cytology of nasal secretions with perennial allegic rhinitis in children : Comparison of non-infected and infected conditions." Laryngoscope. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada T: Extracellular matrix in the field of otolryngology. Extracellular matrix.Sakakura T (ed). Yodosha, Tokyo, 130-141 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 野々山勉、 他: "慢性副鼻腔炎におけるサイトカインの免疫組織学的検討" 免疫アレルギー. 14. 180-181 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 原田輝彦、 他: "鼻茸形成における細胞外マトリックス分解機構の検討" 免疫アレルギー. 15. 40-41 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Guo Y, et al.: "Effects of functional endoscopic sinus surgery on maxillary sinus mucosa" Arch Otolaryngol Head Neck Surg. 123. 1097-1100 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Majima Y, et al.: "Quantitative study of nasal secretory cells in normal subjects and patients with chronic sinusitis" Laryngoscope. 107. 1515-1518 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Harada T, et al.: "The role of extracellular matrix and their degradation system in nasal polyp formation" Am J Rhinol. 11. 412-413 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shinogi J, et al.: "Quantitative cytology of nasal secretions with perennial allergic rhinitis in children:Comparison of non-infected and infected conditions" Laryngoscope. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 原田輝彦(分担執筆): "細胞外マトリックス" 坂倉照好編、羊土社、東京, 172(12) (1995)

    • Related Report
      1997 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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