• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

An investigation of pathogenesis of autoimmuneinner ear disease and an approach for developing new therapeutic strategy

Research Project

Project/Area Number 07671857
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionShimane Medical University

Principal Investigator

KATAOKA Shingo  Shimane Medical Univ.Department of ORL professor, 医学部, 助手 (60152667)

Co-Investigator(Kenkyū-buntansha) SANO Keisuke  Shimane Medical Univ.Department of ORL assistant, 医学部, 助手 (10263542)
KAWAUCHI Hideyuki  Shimane Medical Univ.Department of ORL assistant, 医学部, 教授 (50161279)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsautoimmune inner ear disease / perilymph / inner ear immune response / endolymphatic sac / inner ear development / apoptosis / suppressor factor / 免疫染色 / RT-PCR / 内リンパ水腫 / 神経伝達物質 / 内耳特異抗原 / 内耳自己免疫病 / Tリンパ球 / VIP / SP / 分泌型IgA / 抗体産生細胞 / Secretory component
Research Abstract

To better understand the role of immunocompetent cells in the defense mechanism of the inner ear, recruitments of lymphocyte subsets were quantitatively examined in the perisaccular space(PSS) of rats undergoing a perilymphatic keyhole limpet hemocyanin(KLH) challenge after systemic priming, or passive transfers of KLH-specific serum and/or KLH-primed lymphocytes. The number of IgG-bearing B cells per unit area was significantly higher than that of control side, in the PSS of the endolymphatic sac(ES) on the antigen-challenged side of systemically presensitized rats. In there, helper/inducer and suppressor T cells were also already mobilized one week post inner ear antigen challenge. Antibody titers in perilymph of challenged side were obviously higher than those of control side. Transfer experiments further confirmed that a proliferation and differentiation of antigen-primed B cells with a help of T cells in ES might be essential for antibody production in the inner ear upon antigenic exposure. Functional analyses of resident and recruited T cells in ES from the cytokine profile should be necessary in a future study. In a separate study, the role of apoptosis in the fetal inner ear development was examined, by employing the computer-assisted 3-D reconstruction of the inner ear histology of human embryo and mouse embryo as well. The results indicated the significant role of apoptosis in the inner ear development during fetal stage. The monoclonal non-specific suppressor factor derived from mouse hybirdoma was biochemically examined and the possible clinical application of it for the treatment of autoimmune diseases was introduced.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Hideyuki Kawauchi: "Is endolymphatic sac categorized as the mucosa associated lymphoid tissue (MALT)? -a briefreview on this issue-." Proc.Sendai Symposium. (5). 21-23 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hideyuki Kawauchi: "The role of the endlymphatic sac in inner ear immune response." Proceeding of the 5th international academic workshop on the interaction between middle ear and inner ear. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomoko Nagata: "Conjugation of ubiquitin-like polypeptide to intracellular acceptor proteins." Biochemica et Biophysica Acta. 1401. 319-328 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomoyuki Nishikori: "Apoptosis during inner ear development in human and mouse embryos;an analysis by computer-assisted three-dimensional reconstruction." Embryology and Anatomy. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 川内 秀之: "耳のアレルギー.アレルギーの領域Vol.4 No.2" 医薬ジャーナル社, 23-27 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 川内 秀之: "上気道免疫の老化・治療 Vol.80 No.3" 南山堂, 49-54 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hideyuki Kawauchi: "Is endolymphatic sac categorized as the mucosa associated lymphoid tissue (MALT) ? -a briefreview on this issue-." Proc.Sendai Symposium.(5). 21-23 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hideyuki Kawauchi: "The role of the endlymphatic sac in inner ear immune response." Proceedings of the 5th international academic workshop on theinteraction between middle ear and inner ear. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomoko Nagata, Morihiko Nakamura, Hideyuki Kawauchi, Yoshinori Tanigawa: "Conjugation of ubiquitin-like polypeptide to intracellular acceptor proteins." Biochimica et Biophysica Acta.1401. 319-328 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomoyuki Nishikori, Toshihisa Hatta, Hiroki Otani, Hideyuki Kawauchi: "Apoptosis during inner ear development in human and mouse embryos ; An analysis by computer-assisted three-dimensional reconstruction." Anatomy and Embryology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hideyuki Kawauchi: "Role of ELsac lymphocytes in inner ear immune response" Acta Otolaryngol.Suppl.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 川内秀之: "アレルギーの領域 Vol.4(2)耳のアレルギー" (株)医薬ジャーナル社, (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 川内秀之: "鼻粘膜局所免疫応答におけるリンパ球集簇の役割についての検討" 耳鼻咽喉科免疫アレルギー. 13. 180-181 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hideyuki Kawauchi: "Is endolymphatic sac (ELsac) categorized as the mucosa associated lymphoid tissue (MALT)?-A brief review on this issue-" Sendai Symposium'95. 21-23 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi